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  • Electron microscopic immunocytochemistry  (2)
  • Heterosis  (2)
  • Springer  (4)
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  • Springer  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 637-643 
    Details
    ISSN: 1432-2242
    Keywords: Rice ; Heterosis ; RAPDs ; Microsatellites ; Genetic distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten elite inbred lines (four japonica, six indica), chosen from those widely used in the hybrid rice breeding program at Human Hybrid Rice Research Center in China, were crossed to produce all possible hybrids excluding reciprocals. The 45 F1 hybrids along with the ten parents were evaluated for eight traits of agronomic importance, including yield potential, in a replicated field trial. The ten parents were analyzed with 100 arbitrary decamer oligonucleotide primers and 22 microsatellite (simple sequence repeats, SSRs) primer sets via polymerase chain reaction (PCR). Out of the 100 random primers used, 74 were informative and amplified 202 non-redundant bands (variants) with a mean of 2.73 bands per polymorphic primer. All 22 microsatellite primer sets representing 23 loci in the rice genome showed polymorphisms among the ten parents and revealed 90 alleles with an average of 3.91 per SSR locus. Cluster analysis based on Nei's genetic distance calculated from the 291 (202 RAPDs, 89 SSRs) non-redundant variants separated the ten parental lines into two major groups that corresponds to indica and japonica subspecies, which is consistent with the pedigree information. Strong heterosis was observed in hybrids for most of the traits examined. For the 43 diallel crosses (excluding 2 crosses not heading), yield potential, its components (including panicles per plant, spikelets per panicle and 1000-grain weight) and their heterosis in F1 hybrids showed a significant positive correlation with genetic distance. When separate analyses were performed for the three subsets, yield potential and its heterosis showed significant positive correlations with genetic distance for the 15 indica x indica crosses and the 6 japonica x japonica crosses; however, yield potential and its heterosis were not correlated with genetic distance for the 22 indica x japonica crosses. Results indicated that genetic distance measures based on RAPDs and SSRs may be useful for predicting yield potential and heterosis of intra-subspecific hybrids, but not inter-subspecies hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226083
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 637-643 
    Details
    ISSN: 1432-2242
    Keywords: Key words Rice ; Heterosis ; RAPDs ; Microsatellites ; Genetic distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Ten elite inbred lines (four japonica, six indica), chosen from those widely used in the hybrid rice breeding program at Human Hybrid Rice Research Center in China, were crossed to produce all possible hybrids excluding reciprocals. The 45 F1 hybrids along with the ten parents were evaluated for eight traits of agronomic importance, including yield potential, in a replicated field trial. The ten parents were analyzed with 100 arbitrary decamer oligonucleotide primers and 22 microsatellite (simple sequence repeats, SSRs) primer sets via polymerase chain reaction (PCR). Out of the 100 random primers used, 74 were informative and amplified 202 non-redundant bands (variants) with a mean of 2.73 bands per polymorphic primer. All 22 microsatellite primer sets representing 23 loci in the rice genome showed polymorphisms among the ten parents and revealed 90 alleles with an average of 3.91 per SSR locus. Cluster analysis based on Nei’s genetic distance calculated from the 291 (202 RAPDs, 89 SSRs) non-redundant variants separated the ten parental lines into two major groups that corresponds to indica and japonica subspecies, which is consistent with the pedigree information. Strong heterosis was observed in hybrids for most of the traits examined. For the 43 diallel crosses (excluding 2 crosses not heading), yield potential, its components (including panicles per plant, spikelets per panicle and 1000-grain weight) and their heterosis in F1 hybrids showed a significant positive correlation with genetic distance. When separate analyses were performed for the three subsets, yield potential and its heterosis showed significant positive correlations with genetic distance for the 15 indica×indica crosses and the 6 japonica×japonica crosses; however, yield potential and its heterosis were not correlated with genetic distance for the 22 indica×japonica crosses. Results indicated that genetic distance measures based on RAPDs and SSRs may be useful for predicting yield potential and heterosis of intra-subspecific hybrids, but not inter-subspecies hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050173
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of immunoreactive corticotropin, β-lipotropin, α- and β-endorphin in Cells of the human fetal anterior pituitary (1979)
    Springer
    Cell & tissue research 204 (1979), S. 37-51 
    Details
    ISSN: 1432-0878
    Keywords: Corticotropin ; Opioid peptides ; Anterior pituitary ; Human fetus ; Electron microscopic immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cells immunoreactive with anti-α-(17–39) ACTH, β-(1–24) corticotropin, β-LPH, α- and β-EP were identified in the human fetal anterior pituitary at the ultrastructural level using the peroxidase-antiperoxidase complex method on ultrathin sections. Only one definite cell type was revealed by all these antisera. All granules of each individual immunostained cell reacted regardless of the antiserum used. The immunostained cells occurred in groups and were sometimes located in the wall of the follicle-like structures commonly observed in the fetal anterior pituitary. The cells revealed two main aspects: 1) The largest elements were rich in organelles, and their numerous secretory granules showed significant variations in size (250–500 nm in diameter), electron density of their content and stain-deposit intensity. The ergastoplasm, consisting of irregular tubules, was poorly developed. In the vicinity of the conspicuous Golgi apparatus, organelles related to the GERL complex were commonly observed. Multivesicular bodies were frequent. Some of these cells showed bundles of microfilaments (60 nm in thickness). 2) The smaller cells had an electron-lucent hyaloplasm with sparse organelles; they contained fewer granules and never showed microfilaments. The immunocytological results are consistent with the synthesis of a molecule similar to pro-opiocortin by this type of endocrine cell in human fetuses. Morphological evidence for the maturation process of this precursor and for the secretory activity of these cells and its possible regulation is presented and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235163
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  • 4
    Electronic Resource
    Electronic Resource
    Somatotrophs in the human fetal anterior pituitary (1977)
    Springer
    Cell & tissue research 181 (1977), S. 545-552 
    Details
    ISSN: 1432-0878
    Keywords: Somatotrophs ; Anterior pituitary ; Human fetus ; Electron microscopic immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An immunocytochemical staining of ultrathin sections using the peroxidase-antiperoxidase complex was employed to localize the somatotrophs in the anterior pituitary of the human fetus. This technique allowed a specific identification of the somatotrophs and a detailed description of their ultrastructure which was well preserved by the fixation employed. They appeared as cells with a dense cytoplasmic matrix, lamellar rough endoplasmic reticulum, and prominent Golgi apparatus. Their secretory granules had a mean diameter of 300 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221775
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