ISSN:
1573-6849
Keywords:
chromosomes
;
DNA staining
;
EDX
;
platinum blue
;
scanning electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We describe a DNA-specific staining procedure, using a blue platinum organic dye, which allows DNA imaging of chromosomes by detection of back-scattered electrons in the scanning electron microscope. DNA distribution is visualized within chromosomal details of the centromeric and satellite regions, or in interphase chromatin, with high-resolution (10–15 nm) for comparison with the corresponding secondary electron image representing DNA plus protein.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00710018
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