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Superoxide, hydrogen peroxide, and the gelling of phloem sap from Cucurbita pepo (1982)

McEuen, A. R. ; Hill, H. A. O.

Springer

Planta 154 (1982), S. 295-297

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ISSN: 1432-2048

Keywords: Catalase ; Cucurbita ; Peroxidase ; Phloem sap ; Superoxide dismutase

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The possible involvement of superoxide and hydrogen peroxide in the oxidative gelling of phloem exudate from Cucurbita pepo. was investigated. Neither superoxide dismutase (EC 1.15.1.1) nor catalase (EC 1.11.1.6) inhibited the reaction. Although catalase could not be detected in exudate, both peroxidase (EC. 1.11.1.7) and superoxide dismutase were present in reasonable amounts. Polyacrylamide gel electrophoresis revealed one major and one minor isozyme of superoxide dismutase, both of which were adjudged to contain copper and zinc as their prosthetic metals, on the basis of cyanide inhibition and molecular weight.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00393906

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