ISSN:
1573-6857
Keywords:
Drosophila melanogaster
;
Esterase-6
;
Est-6/Est-P duplication
;
in situ hybridization
;
polytene chromosomes
;
gene mapping
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The Esterase-6 gene locus of Drosophila melanogaster although well-characterized, has not been definitly mapped by in situ hybridization. In this paper, a high resolution in situ hybridization protocol using an avidin/biotinylated-horseradish peroxidase/diaminobenzidine system was adopted to refine the physical map position of the Esterase-6 locus. Clarity of signal, detail of banding pattern and absence of background allowed the assignment of a 1.8 kb cDNA encoding Esterase-6 to three bands within subsections 69 A1–A3 on the left arm of polytene chromosome 3. These data refine earlier deletion mapping and low resolution in situ hybridization results, which assigned Esterase-6 to 69 A1–A5. The potential use of this high resolution in situ hybridization technique in the analysis of the physical organization of the Esterase-6 gene duplication and surrounding region is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00127248
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