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  • Cattle slurry  (1)
  • Cytotoxic T lymphocytes  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 48 (1992), S. 911-920 
    ISSN: 1420-9071
    Keywords: Cytotoxic T lymphocytes ; granules ; perforin ; gene targetting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The killing mediated by cytotoxic T lymphocytes (CTL) represents an important mechanism in the immune defence against tumors and virus infections. The lytic mechanism has been proposed to consist of a polarized secretion of granule-stored molecules, occurring on effector-target cell contact. By electron microscopy, membrane deposited, pore-like lesions are detected on the target cell membrane during cytolysis by CTL. These structures resembled strikingly pores formed during complement attack. Granules of CTL isolated by nitrogen cavitation and Percoll gradient centrifugation were shown to retain cytotoxic activity. Further purification of proteins stored in these granules led to the discovery of a membranolytic protein named perforin which was capable of polymerizing into pore-like structures. In addition to this cytolytic protein, a set of serine esterases was found as well as lysosomal enzymes and proteoglycans, whose function are not yet clearly defined. The role of perforin in the cytotoxic process is currently being explored by ablating the active gene in mice.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1997), S. 442-444 
    ISSN: 1432-0789
    Keywords: Key words Greenhouse effect ; Methane ; Cattle slurry ; Diurnal variations ; Arrhenius equation ; Micrometerorological CH4 emission technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Methane (CH4) emissions from stored slurry were successfully measured by a micrometeorological mass balance technique. Emissions during autumn to late winter ranged from 2 to 100kgCH4-Cha–1day–1. Diurnal variations of CH4 emissions were investigated by measuring emissions at several times during the day, correlated with slurry temperatures at 10cm depth and successfully modelled with the Arrhenius equation.
    Type of Medium: Electronic Resource
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