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  • Cab-gene expression  (1)
  • Evoked potentials  (1)
  • Springer  (2)
  • 1
    ISSN: 1432-2048
    Keywords: Cab-gene expression ; Excitation energy transfer ; Hordeum ; Light harvesting complex I (monoclonal antibody) ; Mutant (barley) ; Thylakoid (freeze-fracture)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nuclear gene mutant chlorina-104 of barley (Hordeum vulgare L.) is chlorophyll-deficient when grown under high irradiance, particularly at low temperatures. Chlorina- 104 chloroplasts had fewer thylakoids than the wild type, and fewer appressed lamellae relative to non-appressed lamellae. The freeze-fracture ultrastructure showed a loss of particles from the protoplasmic fracture face of the stacked thylakoid region (PFs), consistent with the loss of most of the light-harvesting complex (LHC) II, and a loss of some of the large particles from the same face of the unstacked thylakoid region (PFu), indicating a loss of photosystem-I particles. The mutant is remarkable for the high density of particles on the exoplasmic fracture face of the unstacked thylakoid region (EFu), levels of which fell to normal after transfer to low light. The chlorophyll deficiency was shown to be primarily caused by the loss of LHCII and LHCI-680, with the consequent loss of much of the chlorophyll (Chl) b and the xanthophylls neoxanthin and lutein. The use of a monoclonal antibody which recognises the 23-kDa polypeptide of LHCI-680, confirmed that it was severely depleted in chloroplasts from chlorina-104 grown under restrictive conditions. The 77 K fluorescence emission spectrum was characterised by a pronounced shoulder at 720 nm, arising from the photosystem-I reaction centre (CPI). Since fluorescence from CPI is normally quenched by LHCI-730, this indicates that LHCI-680 mediates excitation energy transfer between LHCI-730 and the reaction centre. After moving seedlings to permissive conditions, LHCII and LHCI-680 began to accumulate in the chlorotic leaves and the fluorescence emission spectrum resembled that of wild-type leaves. Measurement of the steady-state mRNA levels with specific Cab probes, showed no difference between wild type and mutant, indicating that control of LHCII and LHCI-680 accumulation was at a post-transcriptional level.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-9686
    Keywords: Biological signal processing ; Electroencephalography ; Evoked potentials ; Spectral estimation ; Phase spectrum ; Coherence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Technology
    Notes: Abstract Techniques for objective response detection aim to identify the presence of evoked potentials based purely on statistical principles. They have been shown to be potentially more sensitive than the conventional approach of subjective evaluation by experienced clinicians and could be of great clinical use. Three such techniques to detect changes in an electroencephalogram (EEG) synchronous with the stimuli, namely, magnitude-squared coherence (MSC), the phase-synchrony measure (PSM) and the spectral F test (SFT) were applied to EEG signals of 12 normal subjects under conventional somatosensory pulse stimulation to the tibial nerve. The SFT, which uses only the power spectrum, showed the poorest performance, while the PSM, based only on the phase spectrum, gave results almost as good as those of the MSC, which uses both phase and power spectra. With the latter two techniques, stimulus responses were evident in the frequency range of 20–80 Hz in all subjects after 200 stimuli (5 Hz stimulus frequency), whereas for visual recognition at least 500 stimuli are usually applied. Based on these results and on simulations, the phase-based techniques appear promising for the automated detection and monitoring of somatosensory evoked potentials. © 2000 Biomedical Engineering Society. PAC00: 8719Nn, 8719Bb
    Type of Medium: Electronic Resource
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