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  • CREST sera  (1)
  • Key words Chromosome elongation  (1)
  • Springer  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Unscheduled apoptosis in meristematic plant cells is triggered via terminal deletions in artificially elongated chromosome arms (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1022-1026 
    Details
    ISSN: 1432-2242
    Keywords: Key words Chromosome elongation ; Deletion ; Mitosis ; Disturbed development ; Fertility ; Apoptosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Chromosomes elongated beyond a critical size by balanced rearrangements reduce the viability and fertility of field bean individuals. The severity of symptoms, ranging from growth retardation to early death of seedlings, increases with the length of the longest chromosome arm. This is paralleled by the incomplete separation of sister chromatids during nuclear division, resulting in chromatin connections between daughter nuclei which become disrupted by cell-wall formation and yield chromatid deletions detectable as micronuclei. By means of the TUNEL assay we show that, compared to the wild-type, a 〉6-fold higher number of meristematic cells of karyotypes with chromosome arms surpassing the limit of tolerance reveal apoptotic nuclei and are prone to die. Thus, terminal chromatid deletions apparently trigger unscheduled apoptosis. Extensive cell death in meristems is eventually responsible for reduced growth, disturbed development and reduced seed set. Differentiated root tissues and microspores did not reveal apoptotic nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050834
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  • 2
    Electronic Resource
    Electronic Resource
    Immunostaining and interphase arrangement of field bean kinetochores (1995)
    Springer
    Chromosome research 3 (1995), S. 27-31 
    Details
    ISSN: 1573-6849
    Keywords: CREST sera ; interphase kinetochore association ; kinetochore ; nuclear proteins ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract More than 100 sera from patients with scleroderma CREST (calcinosis, Raynaud phenomenon, esophageal dismotility, sclerodactyly, telangiectasia) were tested in order to detect antigenic nuclear components of the field beanVicia faba (2n=12). Kinetochores of mitotic chromosomes and prekinetochores of interphase cells from root-tip meristems were specifically labelled via an indirect immunofluorescence procedure by antibodies of one of these sera. In 44% of interphase nuclei in which centromeres could be identified, only half (6) of the number of expected prekinetochores (12) was detected, circumstantially indicating at least transient association of homologous centromeres. Some nuclei showed clustering of centromeres at one pole (Rabl configuration). In metaphase chromosomes, each sister kinetochore contained a fluorescent spot. Western blotting of field bean nuclear proteins revealed four antigenic proteins of 28, 30, 64 and 68 kDa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00711158
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    Paper (German National Licenses)
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