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  • C/1995 O1  (1)
  • Prawn tissues  (1)
  • Springer  (2)
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  • Springer  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Observations of C2 Molecules in the Coma of Comets at Mitaka (1997)
    Springer
    Earth, moon and planets 78 (1997), S. 143-148 
    Details
    ISSN: 1573-0794
    Keywords: C2 ; Production rate ; asymmetrical coma ; C/1996 B2 ; C/1995 O1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract In order to monitor the various phenomena of comets, we continuously made the near-nucleus imaging observations of comets at National Astronomical Observatory, Mitaka. Here we report on the results of the observations of two spectacular comets. One is the analysis of photometry of C2 emission images in the coma of comet Hyakutake (C/1996 B2). By comparing the production rates of C2 radicals with C2H2 molecules, we conclude that the most C2 radicals were originated from C2H2, while some of them might be produced from other parents. The second item is the analysis of the morphology of C2 emission images in the coma of comet Hale-Bopp(C/1995 O1). By applying a ring masking technique, we detected asymmetrical distribution of the C2 molecules. The results of these two comets might suggest that a portion of C2 radicals were formed by the disintegration of the small organic dust grains, like the so-called CHON particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006237210191
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  • 2
    Electronic Resource
    Electronic Resource
    Primary cell cultures isolated from Penaeus monodon prawns (1999)
    Springer
    Methods in cell science 21 (1999), S. 219-223 
    Details
    ISSN: 1573-0603
    Keywords: In vitro ; Cell culture ; Prawn tissues
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have devised a cell culture system for Penaeus monodon prawn cells that uses a defined synthetic medium. Organs were removed from adult prawns ranging in size from 13--19cm rostrum-to-telson length. Cultures consisted of either a blend of hematopoietic and lymphoid cells or ovarian cells. The cells divide rapidly in culture, doubling on average once per week for 5 to 6 weeks. These cultures continue to survive for at least 5 months but the rates of cell division are low after the first 5--6 weeks. In the literature, unicellular eukaryotic marine organisms such as chytrids may contaminate marine cell cultures. In some cases these eukaryotic contaminants may be difficult to distinguish from prawn cells unless detailed ultrastructure or characteristic developmental stages such as zoospores can be observed. Alternatively, we prepared molecular probes from repeated DNA sequences 100--400 bp in length in the P. monodon genome. These species-specific probes were hybridised to genomic DNA from cell culture to confirm proliferation of P. monodon cells in our cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009864212013
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