ISSN:
1432-203X
Keywords:
Key wordsMoricandia arvensis
;
Brassicaceae
;
C3-C4 intermediate
;
Stem explants
;
Somatic embryogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mm 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (〉90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050346
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