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  • Avena (phytochrome)  (5)
  • Springer  (5)
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  • Springer  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    In-vivo phytochrome control of in vitro transcription rates in isolated nuclei from oat seedlings (1984)
    Springer
    Planta 161 (1984), S. 444-450 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Nucleus (transcription) ; Photoinduction (phytochrome) ; Phytochrome (transcription) ; Transcription (phytochrome)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The transcription rate of isolated nuclei from oat seedlings was analysed by measuring the incorporation of radioactive label in trichloroacetic-acid-pelletable material. It was observed that this transcription rate depends on the light pretreatment of the plants: 7.5 h after a 5-min red or far-red light pulse maximum rates were found, but at this time far-red light induced only about half of the stimulation observed after a red light pulse. The far-red reversibility of the effect of the red light pulse indicates that phytochrome controls the capacity for transcription by isolated nuclei. Besides this slow reaction, phytochrome regulated the transcription rates in a very fast response when homogenates were irradiated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394576
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  • 2
    Electronic Resource
    Electronic Resource
    Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy (1990)
    Springer
    Planta 180 (1990), S. 372-377 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Phytochrome (destruction, localisation, sequestering)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intracellular localisation of phytochrome in oat (Avena sativa L. cv. Garry Oat) coleoptiles was analysed by electron microscopy. Serial ultrathin sections of resin-embedded material were indirectly immunolabeled with polyclonal antibodies against phytochrome together with a gold-coupled second antibody. The limits of detectability of sequestered areas of phytochrome (SAPs) were analysed as a function of light pretreatments and amounts of the far-red absorbing form of phytochrome (Pfr) established. In 5-d-old dark-grownAvena coleoptiles SAPs were not detectable if less than 13 units of Pfr — compared with 100 units total phytochrome of 5-d-old dark-grown seedlings — were established by a red light pulse. In other sets of experiments, seedlings were preirradiated either with a non-saturating red light pulse to allow destruction to occur or with a saturating red followed by a far-red light pulse to induce first SAP formation and then its disaggregation. These preirradiations resulted in an increase of the limit of detectability of SAP formation after a second red light pulse to 38–41 and 19–23 units Pfr, respectively. We conclude that with respect to Pfr-induced SAP formation an adaptation process exists and that our data indicate that SAP formation is not a simple self-aggregation of newly formed Pfr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01160392
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  • 3
    Electronic Resource
    Electronic Resource
    Partial purification of sequestered particles of phytochrome from oat (Avenu sativa L.) seedlings (1991)
    Springer
    Planta 183 (1991), S. 265-273 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Phytochrome (sequestering, immunolocalization)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequestered particles of phytochrome (SAPs) were partially purified from red-light-irradiated oat coleoptiles. Phytochrome pelletability was enhanced by using buffers containing 10 mM Mg2+ or high concentrations (0.6–0.8 M) of orthophosphate (Pi). Combining the pelletability of phytochrome in the presence of Mg2+ with that in the presence of 0.6 Pi resulted in a strong enrichment (about 100-fold) of pelletable phytochrome. Antisera were raised against Mg2+-Pi-pellets from darkgrown seedlings. Using these antisera, no evidence was found by Western blotting and immunocytochemistry that SAPs contain major proteins other than phytochrome. The major contamination of these enriched SAP preparations consisted of protein crystals which are probably catalase. The preparations contained methyltransferase and protein-kinase activities which were not associated with SAPs. Phytochrome purified from SAPs served as a substrate for protein-kinase activity but not for the methyltransferase activity. Phytochrome itself did not show any kinase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197798
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  • 4
    Electronic Resource
    Electronic Resource
    Intracellular localisation of phytochrome and ubiquitin in red-light-irradiated oat coleoptiles by electron microscopy (1987)
    Springer
    Planta 171 (1987), S. 332-338 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Phytochrome (localisation, sequestering) ; Ubiquitin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intracellular localisation of phytochrome and ubiquitin in irradiated oat coleoptiles was analysed by electron microscopy. We applied indirect immunolabeling with polyclonal antibodies against phytochrome from etiolated oat seedlings or polyclonal antibodies against ubiquitin from rabbit reticulocytes, together with a goldcoupled second antibody, on serial ultrathin sections of resin-embedded material. Immediately after a 5-min pulse of red light-converting phytochrome from the red-absorbing (Pr) to the far-redabsorbing (Pfr) form-the label for phytochrome was found to be sequestered in electron-dense areas. For up to 2 h after irradiation, the size of these areas increased with increasing dark periods. The ubiquitin label was found in the same electrondense areas only after a dark period of 30 min. A 5 min pulse of far-red light, which reverts Pfr to Pr, given immediately after the red light did not cause the electron-dense structures to disappear; moreover, they contained the phytochrome label immediately after the far-red pulse. In contrast, after the reverting far-red light pulse, ubiquitin could only be visualised in the electron-dense areas after prolonged dark periods (i.e. 60 min). The relevance of these data to light-induced phytochrome pelletability and to the destruction of both Pr and Pfr is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398678
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  • 5
    Electronic Resource
    Electronic Resource
    Intracellular localisation of phytochrome in oat coleoptiles by electron microscopy (1990)
    Springer
    Planta 180 (1990), S. 372-377 
    Details
    ISSN: 1432-2048
    Keywords: Avena (phytochrome) ; Phytochrome (destruction, localisation, sequestering)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The intracellular localisation of phytochrome in oat (Avena sativa L. cv. Garry Oat) coleoptiles was analysed by electron microscopy. Serial ultrathin sections of resin-embedded material were indirectly immunolabeled with polyclonal antibodies against phytochrome together with a gold-coupled second antibody. The limits of detectability of sequestered areas of phytochrome (SAPs) were analysed as a function of light pretreatments and amounts of the far-red absorbing form of phytochrome (Pfr) established. In 5-d-old dark-grown Avena coleoptiles SAPs were not detectable if less than 13 units of Pfr — compared with 100 units total phytochrome of 5-d-old dark-grown seedlings — were established by a red light pulse. In other sets of experiments, seedlings were preirradiated either with a non-saturating red light pulse to allow destruction to occur or with a saturating red followed by a far-red light pulse to induce first SAP formation and then its disaggregation. These preirradiations resulted in an increase of the limit of detectability of SAP formation after a second red light pulse to 38–41 and 19–23 units Pfr, respectively. We conclude that with respect to Pfr-induced SAP formation an adaptation process exists and that our data indicate that SAP formation is not a simple self-aggregation of newly formed Pfr.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198788
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