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  • sunflower  (5)
  • Arabidopsis  (4)
  • FIA  (4)
  • Springer  (13)
  • 1
    Electronic Resource
    Electronic Resource
    Flow-injection spectrophotometric determination of molybdenum and vanadium with carminic acid in the presence of cetyltrimethylammonium bromide (1995)
    Springer
    Microchimica acta 118 (1995), S. 203-211 
    Details
    ISSN: 1436-5073
    Keywords: carminic acid ; molybdenum ; vanadium ; tensoactives ; CTAB spectrophotometry ; FIA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The color-forming reactions of molybdenum(VI) and vanadium(IV) with carminic acid (CA) in the presence of cetyltrimethylammonium bromide (CTAB) were used in the flow-injection determination of these metals. The micellar medium enhances the spectrophotometric features of the Mo(VI)-CA and V(IV)-CA binary complexes, and the higher solubility of these complexes when the CTAB concentration is above critical micelle concentration permits the easy application of flow-injection systems. Under the optimum conditions, linear calibration graphs were obtained from 0.76 to 28.8 μg/ml for molybdenum and from 0.30 to 9.1 μg/ml for vanadium. The detection limits were 0.33 and 0.16 μg/ml for molybdenum and vanadium, respectively. The selectivity of the method was investigated and the procedure was successfully applied to the determination of molybdenum in steels and pharmaceuticals and of vanadium in petroleum products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244360
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  • 2
    Electronic Resource
    Electronic Resource
    Semiautomatic determination of furazolidone and furaltadone by continuous and stopped flow FIA methods (1993)
    Springer
    Microchimica acta 112 (1993), S. 31-39 
    Details
    ISSN: 1436-5073
    Keywords: furazolidone ; furaltadone ; hydrolysis ; FIA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract New methods are proposed for the semiautomatic determination of furazolidone and furaltadone by photometric flow injection or stopped flow FIA analysis, on the basis of their alkaline hydrolysis reactions. Linear calibration graphs between 1 and 30 μg/ml are obtained for both compounds, the detection limits for furazolidone and furaltadone being 0.20 and 0.26 μg/ml, respectively, by flow injection and 0.27 and 0.21 μg/ml by the stopped flow approach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243318
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  • 3
    Electronic Resource
    Electronic Resource
    FIA-Fluorimetric assembly for the determination of noradrenaline hydrochloride by a solid-phase reactor with immobilized hexacyanoferrate(III) (1997)
    Springer
    Microchimica acta 126 (1997), S. 69-72 
    Details
    ISSN: 1436-5073
    Keywords: noradrenaline hydrochloride ; FIA ; solid-phase reactor ; fluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract An FIA assembly provided with immobilized hexacyanoferrate(III) is proposed for the fluorimetric determination of noradrenaline hydrochloride. The oxidative reagent is immobilized by means of a strong anion-exchange resin. The FIA manifold is very simple and the calibration graph is linear over the range 0.5–75mgl−1 noradrenaline hydrochloride with an r.s.d of 0.88% (17 replicates) and a sample throughput of 84h−1. Foreign compounds such as NaCl, sucrose, lactose and sodium sulfate caused no significant errors. The procedure is applied to the determination of noradrenaline in a medicinal formulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01242663
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  • 4
    Electronic Resource
    Electronic Resource
    FIA-fluorimetric determination of adrenaline in pharmaceutical formulations by oxidation with molecular oxygen (1998)
    Springer
    Microchimica acta 128 (1998), S. 187-190 
    Details
    ISSN: 1436-5073
    Keywords: fluorimetry ; FIA ; adrenaline ; pharmaceutical formulations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The fluorimetric determination of adrenaline is carried out in a continuous-flow assembly and by means of the molecular dissolved oxygen. The sample solution merges with an NaOH stream, then the resulting mixture is heated at 73 °C and led to the flow-cell of the fluorimeter. The flow-assembly is very simple and the procedure is quick (107 samples h−1) reproducible (R.S.D. 0.6%), selective and suitable to be applied to determination of adrenaline in formulations. Calibrations graph are linear over the ranges 0.05–15 and 20–40 mgl−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243048
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  • 5
    Electronic Resource
    Electronic Resource
    Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants (1994)
    Springer
    Plant molecular biology 24 (1994), S. 561-569 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis ; ABA mutants ; ABA-responsive genes ; promoter ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abscisic acid-responsive gene rab17 is induced during maize embryo maturation and in vegetative tissues under water stress conditions. To investigate how ABA is involved in the induction of the rab17 gene, we present here a genetic approach to analyse the transcriptional regulation of the 1.3 kb rab17 promoter fragment in transgenic wild-type Arabidopsis and mutants which are deficient (aba) and insensitive (abi1, abi2 and abi3) to ABA. During seed development the rab17 promoter fragment confers similar temporal and spatial regulation on the reporter gene GUS, both in transgenic wild-type and ABA-deficient and ABA-insensitive mutants. The rab17 promoter was only active in embryo and endosperm during late seed development, although the ABA-deficient embryo mutant showed a reduction in the level of GUS activity. During germination rab17 promoter activity decreases, and GUS activity is not enhanced by water stress in transgenic wild-type and mutant plants. In contrast, transcription of the Arabidopsis endogenous rab gene is stimulated by water stress, both in wild-type and ABA-insensitive mutants. Our data suggest that different molecular mechanisms mediate seed-specific expression and ABA water stress induction of the rab17 gene and indicate strong conservation of the seed-specific regulatory mechanism for rab genes in monocot and dicot plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023554
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  • 6
    Electronic Resource
    Electronic Resource
    Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators (1994)
    Springer
    Plant molecular biology 25 (1994), S. 693-704 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis ; cold acclimation ; cold-inducible genes ; freezing tolerance ; 14-3-3 proteins ; protein kinase regulators
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated two Rare Cold-Inducible (RCI1 and RCI2) cDNAs by screening a cDNA library prepared from cold-acclimated etiolated seedlings of Arabidopsis thaliana with a subtracted probe. RNA-blot hybridizations revealed that the expression of both RCI1 and RCI2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. However, RCI1 mRNA accumulates faster and at higher levels than the RCI2 one indicating that these genes have differential responsiveness to cold stress. Additionally, when plants are returned to room temperature, RCI1 mRNA decreases faster than RCI2. In contrast to most of the cold-inducible plant genes characterized, the expression of RCI1 and RCI2 is not induced by ABA or water stress. The nucleotide sequences of RCI1 and RCI2 cDNAs predict two acidic polypeptides of 255 and 251 amino acids with molecular weights of 29 and 28 kDa respectively. The alignment of these polypeptides indicates that they have 181 identical amino acids suggesting that the corresponding genes have a common origin. Sequence comparisons reveal no similarities between the RCI proteins and any other cold-regulated plant protein so far described. Instead, they demonstrate that the RCI proteins are highly homologous to a family of proteins, known as 14-3-3 proteins, which are thought to be involved in the regulation of multifunctional protein kinases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029607
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of Chinese rape mosaic virus (oilseed rape mosaic virus), a crucifer tobamovirus infectious on Arabidopsis thaliana (1996)
    Springer
    Plant molecular biology 30 (1996), S. 191-197 
    Details
    ISSN: 1573-5028
    Keywords: tobamovirus ; Cruciferae ; CRMV ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complete nucleotide sequence of Chinese rape mosaic virus has been determined. The virus is a member of the tobamovirus genus of plant virus and is able to infect Arabidopsis thaliana (L.) Heynh systemically. The analysis of the sequence shows a gene array that seems to be characteristic of crucifer tobamoviruses and which is slightly different from the one most frequently found in tobamoviruses. Based on gene organization and on comparisons of sequence homologies between members of the tobamoviruses, a clustering of crucifer tobamoviruses is proposed that groups the presently known crucifer tobamovirus into two viruses with two strains each. A name change of Chinese rape mosaic virus to oilseed rape mosaic virus is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017814
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  • 8
    Electronic Resource
    Electronic Resource
    A highly repeated DNA sequence in Arabidopsis thaliana (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 417-423 
    Details
    ISSN: 1617-4623
    Keywords: Repetitive DNA ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three members of a family of highly repeated DNA sequences from Arabidopsis thaliana have been cloned and characterized. The repeat unit has an average length of 180 bp and is tandemly repeated in arrays longer than 50 kb. This family represents more than one percent of the Arabidopsis genome. Sequence comparisons with tandemly repeated DNA sequences from other Cruciferae species show several regions of homology and a similar length of the repeat unit. Homologies are also found to highly repeated sequences from other plant species. When the sequence CCGG occurs in the repeated DNA, the inner cytosine is generally methylated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331018
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  • 9
    Electronic Resource
    Electronic Resource
    Sunflower mutant containing high levels of palmitic acid in high oleic background (1997)
    Springer
    Euphytica 97 (1997), S. 113-116 
    Details
    ISSN: 1573-5060
    Keywords: Helianthus annuus ; fatty acids ; palmitic acid ; X-ray mutagenesis ; seed oil ; sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new sunflower mutant, CAS-12, was obtained, which has both high palmitic (≈30%) and high oleic acid contents, and also a substantial amount of palmitoleic acid (≈7%). The mutant was selected after X-ray irradiation of dry seeds of the inbred line BSD-2-423, which had normal palmitic (≈3%) and high oleic (≈88%) acid levels. The increase of palmitic and palmitoleic acids occurred at the expense of the oleic acid content, which decreased to around 55% in respect to the original line. Linoleic acid content is always under 5%. Palmitic and palmitoleic acid levels were similar to those of the high palmitic mutant CAS-5 obtained in a previous programme from a low oleic line isogenic to BSD-2-423 using a similar mutagenic treatment. In that previous programme we also selected three high stearic acid mutants using chemical mutagenic treatment on the same sunflower line (RDF-1-532). We attempted to obtain mutants in other lines but were unsuccessful. The isolation of similar mutants in isogenic parental lines illustrates the importance of the genetic background in the development of specific mutants with an altered seed oil fatty acid composition. The oil of this mutant will increase the range of potential uses of sunflower oil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003045726610
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  • 10
    Electronic Resource
    Electronic Resource
    Genetic analysis of the high oleic acid content in cultivated sunflower (Helianthus annuus L.) (1989)
    Springer
    Euphytica 41 (1989), S. 39-51 
    Details
    ISSN: 1573-5060
    Keywords: Helianthus annuus ; sunflower ; oleic acid content ; linoleic acid content ; genetical analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sunflower lines breeding true for very high oleic acid content in their oil (average levels higher than 85%) were crossed with standard sunflower lines with mean oleic acid levels of 30%. Analysis of the oil of F1 seeds indicated dominance for high oleic levels and control of the genotype of the embryo. Segregating generations were obtained selfing heterozygous high oleic BCnF1 plants from several generations of a backcrossing program to incorporate the high oleic character to standard inbred lines and testcrossing these plants to low oleic material. Analysis of F2 and testcrossed seeds showed three kind of segregations, in both F2 and testcrossed populations, with different proportions of low, intermediate and high oleic types. Genetic analysis of these data supported the hypothesis, that the high oleic character is controlled by three dominant complementary genes OL1, OL2 and OL3. Additional data showing F1 seeds with intermediate oleic content and segregations for high oleic in progenies of intermediate types, suggest the presence of major factors modifying high oleic acid content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022409
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