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  • reproduction  (5)
  • Arabidopsis  (4)
  • FIA  (4)
  • Springer  (13)
  • 1
    Electronic Resource
    Electronic Resource
    A comparison of reproduction, growth and acute toxicity in two populations of Tubifex tubifex (Müller,1774) from the North American Great Lakes and Northern Spain (1996)
    Springer
    Hydrobiologia 334 (1996), S. 199-206 
    Details
    ISSN: 1573-5117
    Keywords: toxicity ; cultures ; oligochaetes ; T. tubifex ; growth ; reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reproduction in Tubifex tubifex is being used as part of a suite of indicators of sediment toxicity in Canada and Spain, and reproduction of T. tubifex is being considered as a component of sediment objectives for environmental regulation and clean-up in the Canadian Great Lakes. The data being used to set these reproductive targets have been developed from a single culture of T. tubifex from Lake Erie. The plasticity of this particular species is well known and before it can be adopted widely as a test organism it is necessary to determine whether a single culture source should be used or if cultures derived from different populations respond similarly. A series of experiments with two cultures, one from Lake Erie the second from a small mountain stream in Northern Spain have shown that the Spanish worms appear to produce fewer cocoons per adult (mean 8.6 S.D. 1.0) than those from Lake Erie (mean 10.4 S.D. 0.3) at 22.5 °C, a standard test temperature. The number of young produced per adult by the Spanish culture is also lower (mean 19.0 S.D. 4.6) than the L. Erie population (mean 30.6 S.D. 2.3), however, the Spanish population has higher reproductions rates at a lower temperature. The Spanish worms also have lower and more variable growth rates than the Canadian population. There also appear to be slight differences in the sensitivities to toxicants, with the Canadian worms having higher LC50s for copper, chromium and cadmium. While there are differences in the responses in the two cultures these are not considered to be sufficient to invalidate the use of either population in a standard bioassay protocol as long as appropriate calibration and validation are undertaken.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017370
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  • 2
    Electronic Resource
    Electronic Resource
    Fecundity, reproduction, and growth of Moina macrocopa fed different algae (1991)
    Springer
    Hydrobiologia 222 (1991), S. 49-55 
    Details
    ISSN: 1573-5117
    Keywords: Moina macrocopa ; survival ; reproduction ; fecundity ; culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three micro algae, Ankistrodesmus convolutus, Scenedesmus incrassatulus and Chlorella vulgaris, at three concentrations, were tested as diets for Moina macrocopa. Their effect on reproduction, fecundity, growth and survival was evaluated. All three algae satisfied the nutritional requirements of M. macrocopa, despite their difference in size. The best concentration, expressed in dry weight, was 5 mg l−1 for all three. Time to first reproduction was 4 days with all diets. Average time between clutches was 33 hours, and maximum number of clutches was 12; the highest average number of offspring per brood was 27. The largest broods were the fourth to sixth, depending on the diet. Ephippium hatching depends on temperature, taking only 24 hours at 30 °C and 48 hours at 27 °C. This species could be an important test organism in aquatic bioassays, and a live food in aquaculture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017499
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of food type and concentration on the survival, longevity, and reproduction of Daphnia magna (1994)
    Springer
    Hydrobiologia 287 (1994), S. 207-214 
    Details
    ISSN: 1573-5117
    Keywords: Daphnia magna ; Ankistrodesmus falcatus ; Scenedesmus incrassatulus ; Cladocera ; reproduction ; culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The freshwater microalgae Ankistrodesmus falcatus and Scenedesmus incrassatulus, at three concentrations (6, 12 and 18 mg l−1 t, dry weight), were tested as diets for Daphnia magna cultured in reconstituted hard water, at 19 ± 1 °C. Effects on survival, and reproductive characteristics were evaluated during a complete life-cycle. Test animals fed with the highest food concentration had the lowest survival, longevity, number of clutches, and average inter-brood times, but the total offspring was similar to that obtained with the lowest food concentration, for the same species of alga. Average longevity ranged from 40 to 85 days, with a minimum of 24 and a maximum of 119 days. The average inter-brood time ranged from 4.2 to 5.8 days, the highest values being observed at the lowest food concentration. The average total offspring was maximum for the intermediate concentrations of both algae. The maximum number of clutches ranged from 9 to 23, with the lowest values being observed at the highest food concentration. Even though both microalgae had similar effects on survival and reproduction, and the greatest differences observed were related to food concentration, it seems that S. incrassalulus is a comparatively better food for D. magna. The most suitable food level should be determined prior to carrying out chronic bioassays, and it is also an important factor in cultures for obtaining neonates for toxicological tests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00010735
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  • 4
    Electronic Resource
    Electronic Resource
    Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators (1994)
    Springer
    Plant molecular biology 25 (1994), S. 693-704 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis ; cold acclimation ; cold-inducible genes ; freezing tolerance ; 14-3-3 proteins ; protein kinase regulators
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated two Rare Cold-Inducible (RCI1 and RCI2) cDNAs by screening a cDNA library prepared from cold-acclimated etiolated seedlings of Arabidopsis thaliana with a subtracted probe. RNA-blot hybridizations revealed that the expression of both RCI1 and RCI2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. However, RCI1 mRNA accumulates faster and at higher levels than the RCI2 one indicating that these genes have differential responsiveness to cold stress. Additionally, when plants are returned to room temperature, RCI1 mRNA decreases faster than RCI2. In contrast to most of the cold-inducible plant genes characterized, the expression of RCI1 and RCI2 is not induced by ABA or water stress. The nucleotide sequences of RCI1 and RCI2 cDNAs predict two acidic polypeptides of 255 and 251 amino acids with molecular weights of 29 and 28 kDa respectively. The alignment of these polypeptides indicates that they have 181 identical amino acids suggesting that the corresponding genes have a common origin. Sequence comparisons reveal no similarities between the RCI proteins and any other cold-regulated plant protein so far described. Instead, they demonstrate that the RCI proteins are highly homologous to a family of proteins, known as 14-3-3 proteins, which are thought to be involved in the regulation of multifunctional protein kinases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029607
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  • 5
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of Chinese rape mosaic virus (oilseed rape mosaic virus), a crucifer tobamovirus infectious on Arabidopsis thaliana (1996)
    Springer
    Plant molecular biology 30 (1996), S. 191-197 
    Details
    ISSN: 1573-5028
    Keywords: tobamovirus ; Cruciferae ; CRMV ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The complete nucleotide sequence of Chinese rape mosaic virus has been determined. The virus is a member of the tobamovirus genus of plant virus and is able to infect Arabidopsis thaliana (L.) Heynh systemically. The analysis of the sequence shows a gene array that seems to be characteristic of crucifer tobamoviruses and which is slightly different from the one most frequently found in tobamoviruses. Based on gene organization and on comparisons of sequence homologies between members of the tobamoviruses, a clustering of crucifer tobamoviruses is proposed that groups the presently known crucifer tobamovirus into two viruses with two strains each. A name change of Chinese rape mosaic virus to oilseed rape mosaic virus is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017814
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  • 6
    Electronic Resource
    Electronic Resource
    Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants (1994)
    Springer
    Plant molecular biology 24 (1994), S. 561-569 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis ; ABA mutants ; ABA-responsive genes ; promoter ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abscisic acid-responsive gene rab17 is induced during maize embryo maturation and in vegetative tissues under water stress conditions. To investigate how ABA is involved in the induction of the rab17 gene, we present here a genetic approach to analyse the transcriptional regulation of the 1.3 kb rab17 promoter fragment in transgenic wild-type Arabidopsis and mutants which are deficient (aba) and insensitive (abi1, abi2 and abi3) to ABA. During seed development the rab17 promoter fragment confers similar temporal and spatial regulation on the reporter gene GUS, both in transgenic wild-type and ABA-deficient and ABA-insensitive mutants. The rab17 promoter was only active in embryo and endosperm during late seed development, although the ABA-deficient embryo mutant showed a reduction in the level of GUS activity. During germination rab17 promoter activity decreases, and GUS activity is not enhanced by water stress in transgenic wild-type and mutant plants. In contrast, transcription of the Arabidopsis endogenous rab gene is stimulated by water stress, both in wild-type and ABA-insensitive mutants. Our data suggest that different molecular mechanisms mediate seed-specific expression and ABA water stress induction of the rab17 gene and indicate strong conservation of the seed-specific regulatory mechanism for rab genes in monocot and dicot plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023554
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  • 7
    Electronic Resource
    Electronic Resource
    Sediment Toxicity Bioassays for Assessment of Contaminated Sites in the Nervion River (Northern Spain). 2. Tubifex tubifex Reproduction Sediment Bioassay (1999)
    Springer
    Ecotoxicology 8 (1999), S. 111-124 
    Details
    ISSN: 1573-3017
    Keywords: Tubifex tubifex ; sediment bioassay ; hormesis ; growth ; reproduction ; egestion rates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Toxicity assessment based on sediment chronic bioassays with the aquatic worm Tubifex tubifex was performed at ten contaminated sites in the industrial area of Bilbao (Northern Spain). One control and three reference sites were also included. Tubifex bioassay measures both survival and reproduction impairment. These endpoints have been contrasted and discussed in relation to somatic growth and both individual and total biomass of cocoons. Survival was only affected at one site which was heavily contaminated by organic compounds, mainly PAHs. A group of four severely ecotoxic sediments was characterised by a drastic reduction in number and size of cocoons, and adult somatic growth. In other group of sediments, some significant increases were found for these variables. It is suggested that these increases represent an effect of hormesis. An index of reproductive effort was used to integrate the relationship between somatic growth and reproduction. Values of reproductive effort at the test sediments were lower than those at the control sediment, suggesting a conservative strategy of oligochaete worms which consisted in an investment into somatic line (growth) at the expenses of offspring. Rates of food consumption which were estimated from egestion rates, were low at the contaminated sites. This fact could be related to the low production levels found at these sites and may reflect avoidance feeding behaviour of the oligochaete worms within the sediments. At some reference sites, high production could have resulted from high nutritional quality of sediments, or to an hormetic effect due to low concentration of some chemicals. Results are discussed in relation to toxicity data from sediment three-brood bioassay with Daphnia magna Straus performed separately on the same sediments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008966702822
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  • 8
    Electronic Resource
    Electronic Resource
    Sediment Toxicity Bioassays for Assessment of Contaminated Sites in the Nervion River (Northern Spain). 1. Three-Brood Sediment Chronic Bioassay of Daphnia Magna Straus (1999)
    Springer
    Ecotoxicology 8 (1999), S. 97-109 
    Details
    ISSN: 1573-3017
    Keywords: Daphnia magna ; sediment toxicity ; reproduction ; hormesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The ecotoxicity of sediments from ten contaminated sites of the Nervion river catchment were assessed with the three-brood chronic sediment bioassay of Daphnia magna. Three additional locations were used as reference sites and one site as control for every bioassay. Survival, number and biomass of neonates and final adult biomass were measured as chronic ecotoxicity endpoints. High mortality of daphnids was observed at one site where sediment was heavily contaminated by metals. At the remaining sites only sublethal reproductive responses were observed, including inhibition of maturity and hence of reproduction, brood delay, reduction in number of neonates and lower adult final biomass. Hormetic responses in adult final biomass and number of neonates per brood were observed where toxicant concentrations were low or were not bioavailable. Female fecundity increased with size in the controls but the reproductive effort is lower in daphnids exposed to contaminated sediments. This was interpreted as an auto-conservative strategy of the species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008914718752
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  • 9
    Electronic Resource
    Electronic Resource
    A highly repeated DNA sequence in Arabidopsis thaliana (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 417-423 
    Details
    ISSN: 1617-4623
    Keywords: Repetitive DNA ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three members of a family of highly repeated DNA sequences from Arabidopsis thaliana have been cloned and characterized. The repeat unit has an average length of 180 bp and is tandemly repeated in arrays longer than 50 kb. This family represents more than one percent of the Arabidopsis genome. Sequence comparisons with tandemly repeated DNA sequences from other Cruciferae species show several regions of homology and a similar length of the repeat unit. Homologies are also found to highly repeated sequences from other plant species. When the sequence CCGG occurs in the repeated DNA, the inner cytosine is generally methylated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331018
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  • 10
    Electronic Resource
    Electronic Resource
    Semiautomatic determination of furazolidone and furaltadone by continuous and stopped flow FIA methods (1993)
    Springer
    Microchimica acta 112 (1993), S. 31-39 
    Details
    ISSN: 1436-5073
    Keywords: furazolidone ; furaltadone ; hydrolysis ; FIA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract New methods are proposed for the semiautomatic determination of furazolidone and furaltadone by photometric flow injection or stopped flow FIA analysis, on the basis of their alkaline hydrolysis reactions. Linear calibration graphs between 1 and 30 μg/ml are obtained for both compounds, the detection limits for furazolidone and furaltadone being 0.20 and 0.26 μg/ml, respectively, by flow injection and 0.27 and 0.21 μg/ml by the stopped flow approach.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243318
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