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  • FIR laser  (3)
  • Animals
  • Coleus (cell cultures)
  • METEOROLOGY AND CLIMATOLOGY
  • Springer  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Purification of rosmarinic acid synthase from cell cultures of Coleus blumei Benth (1993)
    Springer
    Planta 191 (1993), S. 18-22 
    Details
    ISSN: 1432-2048
    Keywords: Coleus (cell cultures) ; Hydroxycinnamic acid ester ; Rosmarinic acid ; Rosmarinic acid synthase (purification)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rosmarinic acid synthase from cell cultures of Coleus blumei Benth. was purified to apparent homogeneity by fractionated ammonium sulfate precipitation (60–80% saturation), hydrophobic interaction chromatography, affinity chromatography and gel filtration. This purification procedure resulted in a 225-fold-enriched specific enzyme activity with a yield of 9%. The protein preparation was apparently pure according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis. The apparent molecular mass determined by gel filtration and SDS-PAGE was 77 kDa, indicating that rosmarinic acid synthase is a monomeric enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240891
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  • 2
    Electronic Resource
    Electronic Resource
    Proposed biosynthetic pathway for rosmarinic acid in cell cultures of Coleus blumei Benth (1993)
    Springer
    Planta 189 (1993), S. 10-14 
    Details
    ISSN: 1432-2048
    Keywords: Coleus (cell cultures) ; Hydroxycinnamic acid ester ; Phenylpropanoid metabolism ; Rosmarinic acid (biosynthetic pathway)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A biosynthetic pathway for rosmarinic acid is proposed. This pathway is deduced from studies of the enzymes detectable in preparations from suspension cells of Coleus blumei. Phenylalanine is transformed to 4-coumaroyl-CoA by the enzymes of the general phenylpropanoid pathway: phenylalanine ammonia-lyase (EC 4.3.1.5), cinnamic acid 4-hydroxylase (EC 1.14.13.11) and hydroxycinnamic acid:CoA ligase (EC 6.2.1.12). Tyrosine is metabolized to 4-hydroxyphenyllactate by tyrosine aminotransferase (EC 2.6.1.5) and hydroxyphenylpyruvate reductase. The ester can be formed from 4-coumaroyl-CoA and 4-hydroxyphenyllactate by the catalytic activity of rosmarinic acid synthase with concomitant release of CoA. Microsomal hydroxylase activities introduce the hydroxyl groups at positions 3 and 3′ of the aromatic rings of the ester 4-coumaroyl-4′-hydroxyphenyllactate giving rise to rosmarinic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201337
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  • 3
    Electronic Resource
    Electronic Resource
    Optically pumped cw CH2DOH fir laser: New lines and frequency measurements (1980)
    Springer
    International journal of infrared and millimeter waves 1 (1980), S. 117-126 
    Details
    ISSN: 1572-9559
    Keywords: CH2DOH ; CO2 laser ; FIR laser ; laser frequency measurement ; new laser lines ; relative intensity ; relative polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have measured the output powers and relative polarizations of 66 cw FIR laser lines from CH2DOH (including 50 not previously reported), which were optically pumped by a CO2 laser. The frequencies of 43 of these lines were measured relative to stabilized CO2 lasers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007071
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  • 4
    Electronic Resource
    Electronic Resource
    Frequencies and wavelengths from a new, efficient FIR lasing gas: CD2F2 (1981)
    Springer
    International journal of infrared and millimeter waves 2 (1981), S. 705-711 
    Details
    ISSN: 1572-9559
    Keywords: CD2F2 ; laser frequency measurement ; FIR laser ; new laser lines ; CO2 laser ; wavelengths ; relative polarization ; relative power
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We report for the first time wavelength, relative polarization, and frequency measurements for 47 new cw FIR laser lines in the wavelength region from 120 to 1714 μm, all obtained by optically pumping CD2F2 with a CO2 laser. Relative output powers were also measured. For comparison, the 189.8 μm line pumped by RI(34) is nearly five times as efficient as the 118.8 μm methyl alcohol line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007273
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  • 5
    Electronic Resource
    Electronic Resource
    Frequency measurements of far infrared cw lasing lines in optically pumped CHCl2F (1983)
    Springer
    International journal of infrared and millimeter waves 4 (1983), S. 401-406 
    Details
    ISSN: 1572-9559
    Keywords: CHCl2F ; laser frequency measurement ; FIR laser ; new laser lines ; CO2 laser ; wavelengths ; relative polarization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The wavelengths, frequencies, and relative polarizations of 15 FIR cw lasing lines obtained by optically pumping CHCl2F with a cw CO2 laser have been measured. The lines are in the wavelength range from 340.3 to 905.4 μm and were pumped by P- and R-branch laser lines in the 9 μm band of CO2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01009169
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