ISSN:
1432-203X
Keywords:
Agrobacterium
;
Somatic Embryogenesis
;
Electroporation
;
β-Glucuronidase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Daucus carota hypocotyl sections were transformed withAgrobacterium tumefaciens LBA4404 containing CaMV 35S promoter, β-glucuronidase coding sequence and the nopaline synthase (Nos) poly adenylation sequences in Bin 19. Sliced sterile seedling hypocotyl segments were preincubated for 2 days, co-cultivated withAgrobacterium for an additional 2 days, and then transferred to medium containing 100ug/ml of kanamycin and 400ug/ml carbenicillin. In 6 weeks kanamycin resistant calli were obtained in 5.8% of the explants from one variety. Calli were subcultured on solid medium, and in 4 weeks introduced into suspension culture. NPTII and Southern blot analysis confirmed that three selected lines were transformed with 1–3 copies of the GUSII construction. GUS activity in transformants was 5 to 250 fold over background.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00716672
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