ISSN:
1617-4623
Keywords:
Streptomyces
;
Selenate resistance
;
Cysteine auxotrophy
;
Sulphate permease
;
ATP sulphurylase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A range of mutants auxotrophic for cysteine (cys) and resistant to selenate (sel) were isolated from many Streptomyces strains but chiefly from S. coelicolor A3(2) and S. lividans 66. Two of the classes of sel/cys mutants probably contained simple biochemical lesions of sulphate permease (selC) and ATP sulphurylase (selA) activities, while a further two classes (selD and selE) were pleiotropic and possibly regulatory. Most classes of sel mutations were clustered around the cysD locus of S. coelicolor. Segments of chromosomal DNA cloned from S. coelicolor, S. cattleya and S. clavuligerus and able to complement various sel/cys mutations allowed the relative positions of these mutations and the cysC and cysD mutations of S. coelicolor to be determined. The sel/cys DNA can be used for two-way selection: Cys+Sels↔Cys-Selr.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00425694
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