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  • Trifolium  (2)
  • cowpea  (2)
  • ASTROPHYSICS
  • Springer  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 15 (1993), S. 45-49 
    ISSN: 1432-0789
    Keywords: Rhizobium ; Trifolium ; Population density ; Soil acidity ; Liming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Clovers are widely used forage legumes on acidic soils in Texas and need inoculation with appropriate rhizobia when first introduced. Acidic soils are not conducive to survival of clover rhizobia. A survey of pastures was undertaken to determine the number of rhizobia present. The effect of liming acidic soils on the survival of clover rhizobia was also evaluated in the laboratory. The number of clover rhizobia was more than 100 cells g-1 soil in 70% of the pastures surveyed but populations within pastures varied by more than two orders of magnitude. The number of years of clover production beyond 1 year did not affect the rhizobial population density. The soil pH of twelve samples was below 5.0 and six samples had populations of rhizobial lower than 100 g-1 soil. Eleven out of sixteen samples from fields that had grown clover and had pH values above 6.0 had populations exceeding 1000 g-1 soil and only three samples had populations lower than 100 g-1 soil. Incubating indigenous or inoculated rhizobia in well-mixed soils having pH values of 5.1 or below resulted in populations declining to below 10 g-1 soil in 6 weeks. Mixing of soils with pH values of up to 5.4 induced reduction of rhizobial numbers, possibly by destroying microsites. Liming of soils to increase pH values above 5.5 improved survival of native or inoculated rhizobia in most cases.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 155-156 (1993), S. 353-354 
    ISSN: 1573-5036
    Keywords: cowpea ; nitrogen fixation ; nitrate ; partitioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract If the quality and quantity of yields from cowpea (Vigna unguiculata [L.] Walp.) are to be maximised, a complete understanding of the N nutrition of the plant must be achieved. The N requirement for developing pods of this species may come from mobilization of N in vegetative tissue, biological N fixation and uptake of N from soil. In this study, the fate of a pulse of fixed 15N2 or of 15NO3-given to different cowpea plants during pod development was determined. The plants were grown in vermiculite in plastic pots that were able to be sealed with silicone adhesive and equipped with a rubber septum so that 15N2 gas could be injected into the air space above the vermiculite, and gas losses would be eliminated. Nineteen days after injection of 15N2 the pods, leaves, nodules and roots contained 65%, 15%, 9%, and 4%, respectively of the quantity of 15N2 fixed. When 15NO3-15N was taken up by other plants during this period, these plant parts contained 40%, 26%, 3% and 19%, respectively, of the total plant 15N. The percentage 15N in roots was greater, and that of 15N in nodules was lower, when 15NO3-15N was applied than when 15N2 was utilised by plants. These results indicate that, while a high percentage of fixed-N or NO3-N given to cowpea plants moved to the developing pods, other sinks were competing for this newly-aquired N.
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  • 3
    ISSN: 1573-5036
    Keywords: cowpea ; green manure ; growth stage ; N mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cowpea [Vigna unguiculata (L). Walp.] has great potential as green manure due to its rapid N accumulation and efficient N2 fixation. The objective of this study was to measure the rate of N mineralization from cowpea plant parts harvested at onset of flowering (5 weeks) and mid pod-fill (7 weeks) under near optimum conditions. Cowpeas were grown in a greenhouse and supplied with 15NH4 15NO3 to isotopically label tissue. Cowpea leaves, stems, and roots were incorporated into a sandy soil (Psammentic Paleustalf) and net N mineralized was measured several times during a 10 week incubation. The amount of N accumulated in 7-week old cowpeas was more than double that in 5-week old cowpeas. The portion of N mineralized after 10 weeks was 24% for 5-week old cowpeas and 27% for 7-week old cowpeas. The rate of N mineralization from leaves and stems increased with plant age, but decreased for roots. The amount of N mineralized from 7-week old cowpeas was more than double (235%) that from 5-week old cowpeas due to greater N accumulation and a more rapid rate of N mineralization of the more mature cowpeas. The greatest amount of N was released from leaves, which amounted to 74 and 65% of total N mineralization from 5- and 7-week old cowpeas, respectively. The percentage of N mineralized by 10 weeks was linearly related to the tissue N concentration of the plant parts and to their C/N ratio. These relationships allow a quick estimation of the amount of N that would mineralize from cowpea residues incorporated into soil based on their N concentration or C/N ratio.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 158 (1994), S. 135-139 
    ISSN: 1573-5036
    Keywords: clover ; inoculation ; nitrogen fixation ; nodulation ; rhizobia ; Trifolium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between numbers of rhizobia and nodulation response of legumes is of considerable practical importance. Experiments were done under controlled conditions to determine the influence of numbers of Rhizobium leguminosarum biovar. trifolii on nodulation of arrowleaf clover (Trifolium vesiculosum Savi.) and crimson clover (T. incarnatum L.). Numbers of rhizobia in excess of 1000 per seed did not substantially increase earliness of nodulation or total number of nodules formed on the taproot. Nodules, however, were formed nearer the top of the taproot as numbers of rhizobia increased to 100,000 per seed. Delayed inoculation experiments indicated that nodulation sites for these clovers only remained susceptible to infection for less than 1 day. Delaying inoculation for 4 days resulted in only a 1 to 2 day delay in nodulation for arrowleaf and crimson clovers respectively and no delay for subterranean clover (T. subterraneum L.). Apparently, larger seedlings nodulated faster.
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