ISSN:
1432-203X
Schlagwort(e):
ABBREVIATIONS ANA: α-Naphthaleneacetic acid; HPLC: high performance liquid chromatography; FWB: fresh weight biomass; LS medium: Linsmaier and Skoog medium; MS: mass spectroscopy; NMR: nuclear magnetic resonance; r2=coefficient of determination; s=standard deviation.
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
SUMMARY Different matrices, obtained by varying calcium (0.1 to 1.5M) and alginate (1 to 1.5%) concentrations, were used to study the influence of immobilisation parameters on the behaviour of S. aviculare. A significant modulation of cell growth, cell release, and scopolin production and excretion has been observed. Physiological and morphological characteristics of Solanum aviculare cells immobilised within Ca-alginate beads were notably different from those of suspended cells. Immobilised S. aviculare have accumulated scopolin (up to 120 μg·g–1 FWB) within beads and excreted it into the culture medium (up to 8 μg·g–1 FWB). Contrary to suspended cells which have accumulated only traces of this metabolite within intracellular compartments (1 μg·g–1 FWB), no scopolin has been found into the culture medium.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01088295
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