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  • allozymes  (2)
  • 7460G  (1)
  • 7460J  (1)
  • Key words Microprojectile bombardment  (1)
  • Springer  (4)
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Keywords
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  • Springer  (4)
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  • 1
    Electronic Resource
    Electronic Resource
    Growth characteristics and transformability of soybean embryogenic cultures (1998)
    Springer
    Plant cell reports 17 (1998), S. 765-772 
    Details
    ISSN: 1432-203X
    Keywords: Key words Microprojectile bombardment ; Mitotic index ; Somatic embryogenesis ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic cultures of soybean [Glycine max (L.) Merr. cv. Jack and Asgrow A2872] were established in liquid Finer and Nagasawa medium, maintained by transfer to fresh medium at biweekly intervals, and subjected to microprojectile bombardment over time. Cultures were not amenable to transformation until they were at least 6 months old. Over time, different cell lines of the same genotype acquired very different culture phenotypes. Histological analysis of cell lines differing in transformation ability showed that the most transformable cultures had cytoplasmic-rich cells in the outermost layers of the tissue. In contrast, the outer layers of less transformable cultures contained cells with prominent vacuoles. Although fresh weight accumulation of the cultures was curvilinear during the 2-week subculture period, a burst of mitotic activity was evident shortly after transfer to fresh medium. This activity usually lasted from the 2nd to the 6th day following subculture, and peaked on the 4th day. Tissues at or near this stage always produced more transient expression of a reporter gene than did bombardments at other times. In addition, the cell lines most amenable to transformation also exhibited the highest mitotic index. Thus any treatment to increase the mitotic index, especially when the cell lines are less than 6 months old, may facilitate the transformation of cell lines from which efficient recovery of transgenic plants is still possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050480
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic evidence for the existence of cryptic species in theAnopheles albitarsis complex in Brazil: Allozymes and mitochondrial DNA restriction fragment length polymorphisms (1993)
    Springer
    Biochemical genetics 31 (1993), S. 97-112 
    Details
    ISSN: 1573-4927
    Keywords: Anopheles albitarsis ; allozymes ; mitochondrial DNA ; restriction fragment length polymorphism ; cryptic species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allozyme and mitochondrial DNA (mtDNA) restriction studies were undertaken to determine the extent of genetic divergence among field populations ofAnopheles albitarsis in Brazil. Two sympatric species,An. deaneorum andAn. marajoara, were identified in collections from Costa Marques (CM), Rondonia. Genetic evidence includes (1) the presence of two types of individuals, each with diagnostic allelic clusters (forHad-1, Pgi-1, Pep-1, Mpi-1, andIdh-1), (2) a deficiency of heterozygotes, and (3) characteristic mtDNA haplotypes. In addition, two allopatric cryptic species ofAn. marajoara were identified, one from Iguape (An. marajoara form IG), Sao Paulo state, and the other from the Island of Marajo (An. marajoara form MA). Though form IG and form-MA resemble form CM in wing spot morphology, they differ from it in diagnostic allozymes and mtDNA haplotypes.An. marajoara form CM had a higher variability (mean heterozygosity,H=0.22, and percentage of polymorphic loci,P=66.7) than did form IG and form MA (H=0.08 in both, andP=25.0 and 33.3, respectively). Form MA and form IG are genetically more similar to each other than both are to form CM. Based on wing morphology, estimates ofF statistics, and genetic similarities, we propose thatAn. albitarsis in Brazil is a species complex. It comprises at least two morphologically distinguishable species: (1)An. deaneorum (currently one taxon) and (2) theAn. marajoara species complex, which further consists of at least three cryptic forms,marajoara form MA,marajoara form IG, andmarajoara form CM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553604
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic evidence for the existence of cryptic species in theAnopheles albitarsis complex in Brazil: Allozymes and mitochondrial DNA restriction fragment length polymorphisms (1993)
    Springer
    Biochemical genetics 31 (1993), S. 97-112 
    Details
    ISSN: 1573-4927
    Keywords: Anopheles albitarsis ; allozymes ; mitochondrial DNA ; restriction fragment length polymorphism ; cryptic species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Allozyme and mitochondrial DNA (mtDNA) restriction studies were undertaken to determine the extent of genetic divergence among field populations ofAnopheles albitarsis in Brazil. Two sympatric species,An. deaneorum andAn. marajoara, were identified in collections from Costa Marques (CM), Rondonia. Genetic evidence includes (1) the presence of two types of individuals, each with diagnostic allelic clusters (forHad-1, Pgi-1, Pep-1, Mpi-1, andIdh-1), (2) a deficiency of heterozygotes, and (3) characteristic mtDNA haplotypes. In addition, two allopatric cryptic species ofAn. marajoara were identified, one from Iguape (An. marajoara form IG), Sao Paulo state, and the other from the Island of Marajo (An. marajoara form MA). Though form IG and form-MA resemble form CM in wing spot morphology, they differ from it in diagnostic allozymes and mtDNA haplotypes.An. marajoara form CM had a higher variability (mean heterozygosity,H=0.22, and percentage of polymorphic loci,P=66.7) than did form IG and form MA (H=0.08 in both, andP=25.0 and 33.3, respectively). Form MA and form IG are genetically more similar to each other than both are to form CM. Based on wing morphology, estimates ofF statistics, and genetic similarities, we propose thatAn. albitarsis in Brazil is a species complex. It comprises at least two morphologically distinguishable species: (1)An. deaneorum (currently one taxon) and (2) theAn. marajoara species complex, which further consists of at least three cryptic forms,marajoara form MA,marajoara form IG, andmarajoara form CM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02399823
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  • 4
    Electronic Resource
    Electronic Resource
    Pinning and vortex dynamics in superconducting (K, Ba)BiO 3 single crystals (1996)
    Springer
    Journal of low temperature physics 105 (1996), S. 1067-1072 
    Details
    ISSN: 1573-7357
    Keywords: 7460J ; 7460G ; 7430C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Pinning and vortex dynamics have been investigated in the 3-dimensional copper free (K, Ba)BiO3superconductor (Tc ∼ 31K) by magnetization and transport measurements up to 30 Tesla. The magnetization curves present a pronounced fishtail effect which persists for time scales down to 10−4s (pulsed field measurements). We show that it is an intrinsic feature of the critical current which can in part be well described by the collective pinning theory. Furthermore, this system presents evidence for a vortex liquid /glass transition for vanishingly small currents. As the current density is increased, dissipation in the glass state is dominated by creep effects. The temperature and current dependence of the activation energy is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00768524
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