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  • Articles  (21)
  • Column liquid chromatography  (8)
  • C-banding  (5)
  • Chromosomal location  (5)
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  • Springer  (21)
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  • Articles  (21)
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  • Springer  (21)
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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of the soluble proteins in grape musts by reversed-phase HPLC (1991)
    Springer
    Chromatographia 32 (1991), S. 463-465 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Grape musts ; Soluble proteins ; Simplex optimization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Reversed-phase high performance liquid chromatography (RP-HPLC) was applied to the analysis of the soluble proteins from grape musts, with protein separation based on hydrophobicity. The initial percentage and rate of increase of organic modifier were optimized using the Modified Simplex method. Both the qualitative and quantitative data in the chromatograms yielded information on the variety origin of grape musts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02327980
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  • 2
    Electronic Resource
    Electronic Resource
    Phosphogluco mutase — a biochemical marker for group 4 chromosomes in the Triticinae (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 555-557 
    Details
    ISSN: 1432-2242
    Keywords: Phosphogluco mutase ; Hexaploid wheat ; Chromosomal location ; Homoeologous group 4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Structural genes for the isozymes of phosphogluco mutase (PGM) (EC 2.7.5.1) have been located on chromosome arms 4Aα, 4BL and 4DS of hexaploid wheat. These results support the homoeologies observed among these chromosome arms and also support the notion of conservation of gene synteny groups within the Triticinae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285013
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  • 3
    Electronic Resource
    Electronic Resource
    The meiotic pairing of nine wheat chromosomes (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 193-198 
    Details
    ISSN: 1432-2242
    Keywords: C-banding ; Heterochromatin ; Meiosis ; Common wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The meiotic identification of nine pairs of chromosomes at metaphase I of meiosis of Triticum aestivum (B genome, 4A and 7A) has been achieved using a Giemsa C-banding technique. As a result, the analysis of the pairing of each chromosome arm in disomic and monosomic intervarietal hybrids between ‘Chinese Spring’ and the Spanish cultivar ‘Pané 247’ could be carried out. Differences in the chiasmata frequencies per chromosome arm cannot be explained on the basis of relative arm lengths only. Possible effects of arm-to-arm heterochromatic differences on meiotic pairing are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272894
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  • 4
    Electronic Resource
    Electronic Resource
    Meiotic pairing of the amphiploid Hordeum chilense X Triticum turgidum conv. durum studied by means of Giemsa C-banding technique (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 85-91 
    Details
    ISSN: 1432-2242
    Keywords: Heterochromatin ; Hordeum chilense ; Tritordeum ; C-banding ; Meiosis ; Triticum ; Amphiploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The meiotic behaviour of the amphiploid Hordeum chilense X Triticum turgidum conv. durum using a C-banding staining method is studied. Nine pairs of chromosomes at metaphase-1 (4A, 7A and the seven of the B genome) were identified and the remaining wheat chromosomes (1A, 2A, 3A, 5A and 6A) and seven of the chilense (1 to 7 H ch chromosomes) were assigned to its particular genome. A similar mean number of univalents from parental genomes (wheat and wild barley) were found. No meiotic pairing between chilense and turgidum chromosomes was detected. Differences in the meiotic behaviour per chromosome and amongst genomes are explained on the basis of cytomorphological and heterochromatin characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264487
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  • 5
    Electronic Resource
    Electronic Resource
    Chromosomal location of isozyme markers in wheat-barley addition lines (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 192-198 
    Details
    ISSN: 1432-2242
    Keywords: Chromosomal location ; Isozyme markers ; Hordeum vulgare L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The peroxidase (CPX, PER), α-amylase (α-AMY), acid and alkaline phosphatase (PHE, PHS) and esterase (EST) zymogram phenotypes of ‘Chinese Spring’ wheat, ‘Betzes’ barley and a number of presumptive ‘Betzes’ chromosome additions to ‘Chinese Spring’ were determined. It was found that five disomic chromosome addition lines could be distinguished from one another and from the other two possible lines on the basis of the zymogram phenotypes of these isozymes. The structural genes Cpxe-H1 and Cpxe-H2 were located in ‘Betzes’ chromosome 1, the Perl-H5 and Perl-H6 in chromosome 2, the α-Amy-H2 and α-Amy-H3 in chromosome 7, the Phs-H5 and Phs-H4 in chromosomes 1 and 3 respectively, the Phe-H2, Phe-H3 and Phe-H4 in chromosome 1, the Phe-H1 in chromosome 3, the Ests-H4, Este-H2 and Ests-H6, Este-H8 in chromosomes 1 and 3 respectively and the Estl-H10 and Estl-H2 structural genes were related to chromosomes 3 and 6 respectively. These gene locations provide evidence of homoeology between ‘Betzes’ chromosomes 1, 2, 3, 6 and 7 and the rye chromosomes 7, 2, 3, 6 and 5, respectively, and also between ‘Betzes’ chromosomes 1, 2, 3, 6 and 7 and the ‘Chinese Spring’ homoeologous groups 7, 2, 3, 6 and 5, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275321
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  • 6
    Electronic Resource
    Electronic Resource
    Biochemical evidence of homoeology between Triticum aestivum and Agropyron intermedium chromosomes (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 826-832 
    Details
    ISSN: 1432-2242
    Keywords: Chromosomal location ; Isozyme markers ; Agropyron intermedium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The alcohol dehydrogenase (ADH), phosphoglucose mutase (PGM), glucosephosphate isomerase (GPI), glutamic oxaloacetic transaminase (GOT), malate dehydrogenase (MDH), leaf esterases (ESTL), leaf acid (ACPH) and endosperm alkaline (PHE) phosphatases, leaf peroxidases (PERL) zymogram phenotypes of Triticum aestivum, Agropyron intermedium, Triticum aestivum — Agropyron intermedium octoploids and six Agropyron intermedium chromosome additions to Triticum aestivum and two ditelocentric addition lines were determined. It was found that the six disomic chromosome addition lines and one ditelocentric chromosome addition line could be distinguished from one another and from the other possible lines on the basis of the zymogram phenotypes of these isozymes. The structural gene Acph-X1 was located on Agropyron chromosome L1, the genes Got-X3 and Mdh-X2 on chromosome L2, the gene Gpi-X1 on chromosome L3, the genes Adh-X1, Pgm-X1 and Phe-3 on chromosome L4, gene Perl-1 on chromosome L5 and the gene Estl-2 on chromosome L7 and chromosome arm L7d2. These gene locations provide evidence of homoeology between Agropyron chromosomes L1, L2, L3, L4, L5 and L7 and the Triticum aestivum chromosomes of homoeologous groups 7, 3, 1, 4, 2 and 6, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266553
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  • 7
    Electronic Resource
    Electronic Resource
    Genetics of rye phosphatases: evidence of a duplication (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 683-689 
    Details
    ISSN: 1432-2242
    Keywords: Secale cereale L ; Phosphatases ; Genetic control ; Chromosomal location ; Linkage maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic analyses were conducted on alkaline phosphatases of the endosperm of dry kernels and leaf acid phosphatases in four open pollinated and one inbred line of cultivated rye (Secale cereale L.). A total of seven alkaline phosphatase isozymes were observed occurring at variable frequencies in the different cultivars analyzed. We propose that at least five loci control the alkaline phosphatases of rye endosperm — Alph-1, Alph-2, Alph-3, Alph-4 and Alph-5 — all of which have monomeric behaviour. The leaf acid phosphatases are controlled by one locus and have a dimeric quaternary structure. All loci coding for alkaline phosphatase isozymes showed one active, dominant allele and one null, recessive allele, except for the locus Alph-3 which showed two active, dominant alleles and one null, recessive one. The linkage analyses suggest the existence of two linkage groups for alkaline phosphatases: one of them would contain Alph-2, Alph-4, Alph-5 and the locus/loci coding isozymes 6 and 7. This linkage group is located in the 7RS chromosome arm. The other group would include Alph-1 and Alph-3 loci, being located in the 1RL chromosome arm. Leaf acid phosphatases have been previously located in the 7RL chromosome arm. Our data also support an independent relationship between loci controlling the endosperm alkaline phosphatases and leaf acid phosphatases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260776
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  • 8
    Electronic Resource
    Electronic Resource
    Location of genes coding isozyme markers on Aegilops umbellulata chromosomes adds data on homoeology among Triticeae chromosomes (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 581-588 
    Details
    ISSN: 1432-2242
    Keywords: Chromosomal location ; Isozyme markers ; Aegilops umbellulata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Zymogram analysis was used to identify the Aegilops umbellulata chromosomes that carry the structural genes for particular isozymes. Wheat, Aegilops and wheat-Aegilops hybrid derivative lines (which contained identified Aegilops chromosomes) were tested by gel electrophoresis for isozymes of particular enzymes. It was found that Aegilops chromosome A (nomenclature according to G. Kimber 1967) carries a structural gene for 6-phosphogluconate dehydrogenase, Aegilops chromosome B carries structural genes for glucose phosphate isomerase and phosphoglucose mutase, Aegilops chromosome D carries genes for leaf peroxidases, Aegilops chromosome E carries structural genes for endosperm peroxidases, acid phosphatases and leaf esterases, Aegilops chromosome F carries a gene for embryo plus scutellum peroxidases and Aegilops chromosome G carries structural genes for endosperm alkaline phosphatases, leaf alkaline phosphatases and leaf esterases. The results obtained indicate that chromosome B is partially homoeologous of the wheat chromosomes of group 1 and 4, and chromosome E is partially homoeologous of wheat chromosomes of groups 7 and 4. Circumstantial evidence is also provided about the possible association between chromosomes C, D and A of A. umbellulata respectively with chromosomes 5, 2 and 1 of wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289198
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  • 9
    Electronic Resource
    Electronic Resource
    Identification of C-banded chromosomes in meiosis of common wheat, Triticum aestivum L. (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 257-261 
    Details
    ISSN: 1432-2242
    Keywords: C-banding ; Common wheat ; Triticum aestivum L. ; Translocations ; Heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The C-banding pattern of nine meiotic chromosomes of common wheat (Triticum aestivum L.) as described. In F1s of crosses between monosomics of ‘Chinese Spring’ and two Spanish wheat cultivars, univalent chromosomes were used to aid the recognition and analysis of the C-banding pattern for the individual chromosomes. The identification of one chromosome involved in one translocation in ‘Chinese Spring’ x ‘Pané 247’ has been made through heterochromatin bands observed in the chromosomes involved in multivalents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317049
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  • 10
    Electronic Resource
    Electronic Resource
    Gel filtration and high-performance liquid chromatographic analysis of phosphotungstic acid soluble peptides from blue cheeses (1987)
    Springer
    Chromatographia 23 (1987), S. 764-766 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Gel permeation chromatography ; Cheese peptides ; Amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The nitrogen fraction from cheese, soluble in phosphotungstic acid has been analyzed by gel permeation and high performance liquid chromatography. Elution profiles of this fraction on Sephadex G-10 show that there are no peptides of molecular weight higher than 700 daltons. Six fractions have been obtained. The first one has the peptides and some amino acids. Fractions II to VI contains mostly free amino acids. Fraction I has been separated into many peaks by HPLC. Six or seven of these peaks are presumed to correspond to peptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02312670
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