ISSN:
1573-5001
Keywords:
Heteronuclear coupling
;
Cyclic glucan
;
13C-filtered 2D {1H,1H} NMR
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary A method for measuring three-bond 13C-1H scalar coupling constants across glycosidic bonds in a cyclic β(1→2)-glucan icosamer is presented. This oligosaccharide molecule, with its high degree of symmetry, represents a particular challenge for NMR spectroscopy to distinguish inter-residue from intra-residue heteronuclear coupling effects. Chemically equivalent H2 protons in adjacent glucosyl residues are distinguished on the basis of their different through-space, dipolar interactions with the anomeric protons (H1). The strong NOE contact between anomeric (H1) and aglyconic (H2′) protons permits the selective observation of the inter-residue heteronuclear couplings 3JC1H2′ and 3JC2′H1 in a natural-abundance 13C-ω1-half-filtered {1H,1H} ROESY experiment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00242477
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