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  • Chemistry  (4)
  • 2-n-heptyl-4-hydroxyquinoline-N-oxide  (1)
  • Anaerobic respiration  (1)
  • Astrophysics
  • Wiley-Blackwell  (4)
  • Springer  (2)
Collection
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  • Wiley-Blackwell  (4)
  • Springer  (2)
Years
  • 1
    ISSN: 1432-072X
    Keywords: Photosynthetic bacteria (Rhodobacter capsulatus) ; Transposon mutagenesis ; TMAO reduction ; DMSO reduction ; Anaerobic respiration ; Electron transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1) Rhodobacter capsulatus (formerly Rhodopseudomonas capsulata) strain 37b4 was subjected to transposon Tn5 mutagenesis. 2) Kanamycin-resistant transconjugants were screened for their inability to reduce trimethylamine-N-oxide (TMAO) as judged by the lack of alkali production during anaerobic growth on plates containing glucose as carbon source and cresol red as pH indicator. 3) Of 6 mutants examined, all were found to have considerably decreased levels of methylviologen-dependent TMAO reductase activity and dimethylsulphoxide (DMSO) reductase activity. 4) Periplasmic fractions of one of these mutants (DK9) and of the parent strain were subjected to sodium dodecylsulphate polyacrylamide gel electrophoresis. The gels were stained for TMAO-reductase and DMSO-reductase. With the wild-type strain, only a single polypeptide band, Mr=46,000, stained for TMAO and DMSO reductase activity. In mutant DK9 this band was not detectable. 5) In contrast to the parent strain, harvested washed cells of mutant DK9 were unable to generate a cytoplasmic membrane potential in the presence of TMAO or DMSO under dark anaerobic conditions. 6) In contrast to the parent strain, DK9 was unable to grow in dark anaerobic culture with fructose as the carbon source and TMAO as oxidant.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-072X
    Keywords: Rhodobacter capsulatus ; Nitrate reduction ; Auxiliary electron transport ; Myxothiazol ; 2-n-heptyl-4-hydroxyquinoline-N-oxide ; Ubiquinone pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of various electron transport inhibitors upon the rates of reduction NO 3 - , dimethyl sulphoxide (DMSO) and N2O in anaerobic suspensions of Rhodobacter capsulatus have been studied. A new method for the determination of the rates of reduction of these auxiliary oxidants in intact cells is presented, based on the proportionality observed between the concentration of oxidant and the duration of the electrochromic carotenoid bandshift. For NO 3 - and N2O good agreement was found between rates of reduction determined using electrodes and those determined by the electrochromic method. Myxothiazol and antimycin A had no effect on the rates of reduction of NO 3 - and DMSO suggesting that the cytochrome b/c 1complex is not involved in electron transport to these oxidants. 2-n-heptyl-4-hydroxyquinoline-N-oxide (HOQNO) inhibited at two sites, one within the cytochrome b/c 1complex and the other on the nitrate reducing pathay, but had no effect on electron transport to N2O or DMSO. In both intact cells and cell free extracts, HOQNO had no effect on the nitrate dependent re-oxidation of reduced methylviologen (MVH2), a direct electron donor to nitrate reductase. Our data are consistent with a branch point for the auxiliary electron transport pathways at the level of the ubiquinone pool.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 6 (1995), S. 237-242 
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Because of the recent trend in total hip arthroplasty to achieve direct contact of the endoprosthesis with cortical bone, comparison of bone apposition from cortical bone and cancellous bone was performed. The response of cortical and cancellous bone to hydroxylapatite was also compared. Implants in cortical bone demonstrated a higher interface shear strength and more bone apposition than those in cancellous bone over all time periods tested. Hydroxylapatite coating increased the interfacial shear and bone apposition in cortical bone at all time periods and in both the 3- and 6-week cancellous bone models. The potential effect of stress shielding (and subsequent bone resorption) by femoral stems ingrown with cortical bone is discussed. © 1995 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 22 (1988), S. 119-123 
    ISSN: 0021-9304
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0730-6679
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The dry erosion behavior of epoxy/waste rubber composites has been investigated as a function of rubber content, air pressure (particle velocity), and impact angle. Morphological aspects have also been examined. The experimental data have been explained by means of an analytic equation derived from the crack propagation physics.
    Additional Material: 12 Ill.
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 2 (1960), S. 299-311 
    ISSN: 0368-1467
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A system is described which uses photosynthesis to regenerate oxygen from exhaled carbon dioxide in sealed cabins in the absence of gravity. Design considerations as to choice of algal strain, illumination, nutrients and gas-exchange methods are discussed. Details are given of such an apparatus, of laboratory size, used to evaluate selected semipermeable gas-exchange membranes. Data is presented and extrapolated to estimate size and weight of a possible manned space system.
    Additional Material: 6 Ill.
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