ISSN:
1573-0603
Keywords:
colonic mucosa
;
feeder layer
;
epithelium
;
long-term culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Many attempts have been made to culture the stem cells that are known to reside at the base of the intestinal crypt. However, no successful method for the long-term culture of these cells has yet been published. We wish to describe a method that maintains human colonic crypt cells in a viable, slowly proliferative state for more than 3 mo. The method involves the use of a viable bovine aortic endothelial cell feeder layer that limits its potential for the study of potential growth factors for the colonic cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01666139
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