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  • 1
    Publication Date: 2012-07-24
    Description: Many pathological states involve dysregulation of mitochondrial fusion, fission, or transport. These dynamic events are usually studied in cells lines because of the challenges in tracking mitochondria in tissues. To investigate mitochondrial dynamics in tissues and disease models, we generated two mouse lines with photo-activatable mitochondria ( PhAM ). In the PhAM floxed line, a mitochondrially localized version of the photo-convertible fluorescent protein Dendra2 (mito-Dendra2) is targeted to the ubiquitously expressed Rosa26 locus, along with an upstream loxP -flanked termination signal. Expression of Cre in PhAM floxed cells results in bright mito-Dendra2 fluorescence without adverse effects on mitochondrial morphology. When crossed with Cre drivers, the PhAM floxed line expresses mito-Dendra2 in specific cell types, allowing mitochondria to be tracked even in tissues that have high cell density. In a second line ( PhAM excised ), the expression of mito-Dendra2 is ubiquitous, allowing mitochondria to be analyzed in a wide range of live and fixed tissues. By using photo-conversion techniques, we directly measured mitochondrial fusion events in cultured cells as well as tissues such as skeletal muscle. These mouse lines facilitate analysis of mitochondrial dynamics in a wide spectrum of primary cells and tissues, and can be used to examine mitochondria in developmental transitions and disease states. © 2012 Wiley Periodicals, Inc.
    Topics: Biology , Medicine
    Published by Wiley
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  • 2
    Publication Date: 2011-07-29
    Description: Agricultural census data have been identified as possessing the potential to provide constraints on modeled carbon uptake by croplands at the regional scale. In this study, we build on previous efforts and further assess this potential quantitatively by comparing (1) fractional cropland coverage in southwestern Ontario, Canada, derived from agricultural statistics against three different remotely sensed land cover maps and (2) carbon uptakes determined from agricultural data with simulations generated by a satellite-data-driven biospheric model. In addition, we assimilated the census-data-derived carbon uptakes with modeled estimates in a Bayesian inverse approach to determine if the crop data can provide constraint, as exhibited by uncertainty reductions, and if so, how much. Uncertainties in census-data-derived gross primary production (GPP) estimates are carefully quantified using a Monte Carlo simulation. In general, results from the fractional cropland coverage comparison indicate significant value of the agricultural census data by revealing biases in the spatial distribution of croplands, as found in all three of the satellite land cover products. However, we find that the carbon uptake values derived from crop harvested records are still subject to significant uncertainties that have been underestimated or neglected altogether in past studies. The Monte Carlo simulation suggests that the largest source of uncertainty can be traced to errors in the growth efficiency, followed by harvest production records, and then the harvest index. As a result, attention must be paid to such errors when using the agricultural census data for carbon accounting purposes or to provide constraints to simulations of crop carbon uptake.
    Print ISSN: 0148-0227
    Topics: Biology , Geosciences
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 3
    Publication Date: 2011-04-20
    Description: The European Centre for Medium-Range Forecasts Reanalysis-40 and National Centers for Environmental Prediction (NCEP)/Department of Energy reanalyses are downscaled over the eastern Caribbean and Lesser Antilles using the NCEP-Scripps Regional Spectral Model for Augusts when the Atlantic Warm Pool (AWP) area is the most anomalous. The simulations show a two-way influence between the Lesser Antilles and the AWP: the islands modulate the regional atmospheric circulation, and AWP variations modulate the interannual variabilities of the islands. The Lesser Antilles introduce diurnal variations and drag to the easterlies. The presence of the islands modulates the prevalent easterlies as a result of the daytime heating of the islands and the consequent boundary layer expansion. The modulations are sensitive to the islands' size and topography. Small and flat islands act as thermal plumes, but the modulations of large and hilly islands are during the daytime as their boundary layer expands. The manifestation of the atmospheric response to the sea surface temperature (SST) anomalies over the islands is sensitive to the island orography. For most islands, the atmospheric response to the SST anomalies is reflected only during the daytime. For all one-grid-point islands and Antigua, nighttime and dawn minimum temperatures are modulated to the same degree as the daytime maximum. For island rainfall, downscaling reduces the gross overestimations of rainfall in the reanalyses. However, our downscaling results suggest that there is room for improvement in simulating the marine surface diurnal cycle.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 4
    Publication Date: 2016-04-25
    Description: ABSTRACT Sub-daily rainfall extremes may be associated with flash flooding, particularly in urban areas but, compared with extremes on daily timescales, have been relatively little studied in many regions. This paper describes a new, hourly rainfall dataset for the UK based on ∼1600 rain gauges from three different data sources. This includes tipping bucket rain gauge data from the UK Environment Agency (EA), which has been collected for operational purposes, principally flood forecasting. Significant problems in the use of such data for the analysis of extreme events include the recording of accumulated totals, high frequency bucket tips, rain gauge recording errors and the non-operation of gauges. Given the prospect of an intensification of short-duration rainfall in a warming climate, the identification of such errors is essential if sub-daily datasets are to be used to better understand extreme events. We therefore first describe a series of procedures developed to quality control this new dataset. We then analyse ∼380 gauges with near-complete hourly records for 1992–2011 and map the seasonal climatology of intense rainfall based on UK hourly extremes using annual maxima, n -largest events and fixed threshold approaches. We find that the highest frequencies and intensities of hourly extreme rainfall occur during summer when the usual orographically defined pattern of extreme rainfall is replaced by a weaker, north–south pattern. A strong diurnal cycle in hourly extremes, peaking in late afternoon to early evening, is also identified in summer and, for some areas, in spring. This likely reflects the different mechanisms that generate sub-daily rainfall, with convection dominating during summer. The resulting quality-controlled hourly rainfall dataset will provide considerable value in several contexts, including the development of standard, globally applicable quality-control procedures for sub-daily data, the validation of the new generation of very high-resolution climate models and improved understanding of the drivers of extreme rainfall.
    Print ISSN: 0899-8418
    Electronic ISSN: 1097-0088
    Topics: Geosciences , Physics
    Published by Wiley
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  • 5
    Publication Date: 2005-04-01
    Print ISSN: 0169-3913
    Electronic ISSN: 1573-1634
    Topics: Geosciences , Technology
    Published by Springer
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  • 6
    Publication Date: 2012-03-08
    Print ISSN: 0930-7575
    Electronic ISSN: 1432-0894
    Topics: Geosciences , Physics
    Published by Springer
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 144 (1995), S. 199-208 
    ISSN: 1432-1424
    Keywords: Channel reconstitution ; Ca2+-release channel ; Paramecium ; Liposome ; Internal membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Toward isolating channel proteins from Paramecium, we have explored the possibility of functionally reconstituting ion channels in an artificial system. Proteins from Paramecium cortex reconstituted with soybean azolectin retained several channels whose activities were readily registered under patch clamp. The most commonly encountered activities were three: (i) a 71-pS cation channel that opens at all voltages unless dior trivalent cations were added to close them, (ii) a 40 pS monovalent cation channel, and (iii) a large-conductance channel that prefers anions and exhibits many subconductance states. These channels survived mild detergent treatments without observable functional alterations. The possible origin of these channels from internal membranes, the possible role of 71-pS channel in internal Ca2+ release, and the prospects of their purification are discussed.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 44 (1996), S. 582-588 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Xanthobacter Py2 grows on propene as sole carbon source, converting propene to propene oxide (epoxypropane) using an alkene-specific monooxygenase, as the first step in catabolism. Four mutants, NZ1–4, with a propene- propene oxide+ phenotype were isolated by 1-methyl-3-nitro-1-nitroso-guanidine mutagenesis or by enrichment with the suicide substrate vinylidene chloride, and were shown to have lost the ability to convert alkenes to epoxides. All four mutants were complemented by a number of clones of Xanthobacter Py2 chromosomal DNA in the broad-host-range cosmid pLAFR5, some of which appeared to be non-overlapping. Representatives of the different clones obtained were transferred into Xanthobacter autotrophicus JW33 and one, pNY2, the most frequently isolated clone, was shown to express an inducible, fully functional propene monooxygenase. Subcloning revealed that all four mutants were complemented by a 2.4-kb EcoRI-PstI fragment situated at one end of the cosmid insert. However, activity in X. autotrophicus JW33 could only be expressed from pNY2, containing the complete insert (25 kb), suggesting a large operon or some form of long-range control. pNY2 failed to express in E. coli. In X. autotrophicus JW33 [pNY2] at least three new polypeptides were evident after induction with propene compared with a control carrying only the cosmid pLAFR5.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Computational mechanics 24 (2000), S. 491-502 
    ISSN: 1432-0924
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract  An improved stochastic separated flow model is proposed to obtain reasonable statistical characteristics of a two-phase flow. Effects of the history of a particle and its current trajectory position on the mean-square fluctuating velocity of the dispersed phase are continuously considered in this model. Comparing with the conventional model, results using the improved model are more reasonable and can also be obtained more easily. Furthermore, the improved model requires less computational particles for simulating dispersed-phase turbulence at the beginning of the stochastic trajectory. In this paper, an application in turbulent two-phase flow of planar mixing layer is carried out. Numerical results including velocity, mean-square fluctuating velocity, particle number density and pdf of fluctuation velocity of dispersed phase are shown to compare well with experimental data.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 59 (1997), S. 708-714 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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