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  • 1
    Electronic Resource
    Electronic Resource
    Quantification of thigmotropism (contact sensing) of Candida albicans and Candida tropicalis (1997)
    Springer
    Mycopathologia 138 (1997), S. 13-19 
    Details
    ISSN: 1573-0832
    Keywords: Candida albicans ; Candida tropicalis ; germ tube ; hypha ; thigmotropism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract To quantify the thigmotropism, we adapted the our previous method using a chemotaxifilter system in combination with a bioluminescent adenosine triphosphate (ATP) assay based on firefly luciferase-luciferin system and analyzed the relationship between the ability of germ tube formation and thigmotropism of C. albicans and C. tropicalis. Both the ability to form germ tube and the amount of hyphae exhibiting thigmotropism varied depending upon both the species and strains of Candida. C. albicans formed more germ tubes than C. tropicalis. A good correlation was observed between the ability to form a germ tube and the capacity for thigmotropism, and the results gave a level of significance (p〈0.05). Further, SEM observation revealed that relatively long hyphae of C. tropicalis with penetrated through the pores of filter membrane. This phenomenon may be of importance in the development of pathogenesis of C. tropicalis as well as C. albicans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006849532064
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  • 2
    Electronic Resource
    Electronic Resource
    The NF-κB binding site is essential for transcriptional activation of the IL-15 gene (1998)
    Springer
    Immunogenetics 48 (1998), S. 1-7 
    Details
    ISSN: 1432-1211
    Keywords: Key words Interleukin-15 ; Gene transcription ; Macrophage ; NF-κB ; LPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We cloned the 5’ upstream region of IL-15 genomic DNA and examined promoter activity in macrophages stimulated with lipopolysaccharide(LPS). The 1.2 kilobase (kb) fragment of the 5’ upstream region contained binding elements for LPS-inducible transcription factors such as NFIL-6 or NF-κB. Determined by luciferase assay following transient transfection in the J774A.1 macrophage cell line, the 1.2 kb of the 5’ upstream region exhibited high promoter activity in response to LPS, while promoter activity was significantly reduced by the 5’ deletion of 313 base pairs containing the NF-κB binding motif. Nuclear protein prepared from LPS-stimulated macrophages formed a complex with the NF-κB binding sequence of the IL-15 promoter. Taken together, the binding of nuclear protein to the NF-κB binding site is required for transcriptional activation of the IL-15 gene in LPS-stimulated macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050393
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