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  • 4-methoxyindole-3-acetonitrile  (1)
  • LPS  (1)
  • Springer  (2)
  • Wiley
  • 1
    Electronic Resource
    Electronic Resource
    The NF-κB binding site is essential for transcriptional activation of the IL-15 gene (1998)
    Springer
    Immunogenetics 48 (1998), S. 1-7 
    Details
    ISSN: 1432-1211
    Keywords: Key words Interleukin-15 ; Gene transcription ; Macrophage ; NF-κB ; LPS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  We cloned the 5’ upstream region of IL-15 genomic DNA and examined promoter activity in macrophages stimulated with lipopolysaccharide(LPS). The 1.2 kilobase (kb) fragment of the 5’ upstream region contained binding elements for LPS-inducible transcription factors such as NFIL-6 or NF-κB. Determined by luciferase assay following transient transfection in the J774A.1 macrophage cell line, the 1.2 kb of the 5’ upstream region exhibited high promoter activity in response to LPS, while promoter activity was significantly reduced by the 5’ deletion of 313 base pairs containing the NF-κB binding motif. Nuclear protein prepared from LPS-stimulated macrophages formed a complex with the NF-κB binding sequence of the IL-15 promoter. Taken together, the binding of nuclear protein to the NF-κB binding site is required for transcriptional activation of the IL-15 gene in LPS-stimulated macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050393
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  • 2
    Electronic Resource
    Electronic Resource
    Allelopathy of yellow fieldcress (Rorippa sylvestris): Identification and characterization of phytotoxic constituents (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 683-691 
    Details
    ISSN: 1573-1561
    Keywords: Allelopathy ; Rorippa sylvestris ; weeds ; salicylic acid ; p-hydroxybenzoic acid ; vanillic acid ; syringic acid ; hirsutin ; 4-methoxyindole-3-acetonitrile ; pyrocatechol ; root exudate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Both the neutral and acidic fractions of the acetone extract of yellow fieldcress (Kireha-inugarashi,Rorippa sylvestris Besser) inhibited lettuce seed germination. Salicylic,p-hydroxybenzoic, vanillic, and syringic acid were identified in the acidic fraction. In the neutral fraction, hirsutin (8-methylsulfinyloctyl isothiocyanate), 4-methoxyindole-3-acetonitrile, and pyrocatechol were identified. Bioassay using a root exudate recirculating system showedR. sylvestris during flowering inhibited the lettuce seedling growth. Hirsutin (13μg/plant/day) and pyrocatechol (9.3μg/plant/day) were the major compounds released into the rhizosphere. Several combinations of pyrocatechol,p-hydroxybenzoic acid, vanillic acid, and hirsutin reduced lettuce seedling growth. These compounds seemed to be allelochemicals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00994606
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    Paper (German National Licenses)
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