Publication Date:
2024-04-20
Description:
This dataset provides operational taxonomic units (OTU) abundance table of soil fungi with assigned taxonomic and functional guilds. The soil samples were collected in pure and beech-conifer mixed tree stands in Germany in November/December 2017, at 10 cm depth after removal of non-decomposed litter layer. The tree stands and site descriptions are provided (Ammer et al., 2020; Glatthorn, 2020). DNA was extracted from the samples using the DNeasy® PowerSoil® Pro kit (Qiagen, Hilden, Germany) and purified with DNeasy® PowerClean® kit (Qiagen, Hilden, Germany). Amplification (PCR) was achieved by using primers Mi_ITS3KYO2 (1:10) (Toju et al., 2012) and Mi_ITS4 (1:10) (White et al., 1990). The purification, pooling and quantification of PRC reactions were done as described (Clausing et al., 2020). Illumina sequencing was conducted by the Göttingen Genomics Laboratory (G2L) on MiSeq Platform using MiSeq Reagent Kit v3 (Illumina Inc., San Diego, USA). Raw paired - end reads (fasta.gz files: forward and reverse) were analyzed with MiSeq Marker gene pipeline V1.9. The abundance table contains 97% similarity fungal sequences rarefied to same number of counts (13668). Functional annotation of fungi was done using the FUNGuild database (Nguyen et al., 2016). The raw data were deposited in NCBI (National Center for Biotechnology Information) SRA (Sequence Read Archive) with bioproject accession number: PRJNA704813.
Keywords:
beech-conifer stands; Binary Object; Binary Object (File Size); Binary Object (Media Type); Dassel; Enrichment of European beech forests with conifers; functional guilds; Germany; Goehrde_I; Goehrde_II; Harz; Nienburg; Nienover; OTU abundance; RTG2300; RTG2300-I; soil fungi; Unterluess; VID; Visual identification; Winnefeld
Type:
Dataset
Format:
text/tab-separated-values, 2 data points
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