ALBERT

Beschreibung: In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) the antioxidant activity (AA) experiment aimed to test a previously selected strain, Ulva lacinulata from the NE-Atlantic, and evaluate the variations in AA under different salinity treatments. We hypothesized that by reducing the salinity level in the recirculating land-based system, it would be possible to optimize and increase the quality of the biomass being produced before it being harvested. The NE-Atlantic seaweed was collected in the Óbidos Lagoon, Portugal in January 2021 and was cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany. To recreate the conditions of a large-scale system, the strain was grown previously and during the experiment in a medium of artificial seawater (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) enriched with a cheaper commercial fertilizer Blaukorn (COMPO SANA®, Germany). Twelve 1 L beakers were filled with artificial seawater at 4 different salinities (10, 15, 20 and 30 PSU), measured with a refractometer (Atago, Japan) (n = 3). Six discs (2 cm) of U. lacinulata were placed in each beaker and, at different times (0 h, 3 h, 24 h, 120 h, 192 h and 240 h) 1 disc was collected from each beaker to evaluate the antioxidant activity. The AA was determined by the ABTS Radical Cation Decolourisation Assay based on Re et al., 1999, and a Trolox standard curve was created by measuring the absorption of different Trolox concentrations in ethanol after being mixed with ABTS. The absorption of the samples at 734 nm was analysed with a microplate reader (Infinite 200 Microplate Reader, Tecan Trading AG, Männedorf, Switzerland) and the AA results obtained were given in Trolox Equivalent (in µg/mL).

Beschreibung: Conductivity-temperature-depth profiles were measured using a Seabird SBE 911plus CTD during RV HEINCKE cruise HE585 between 2021-09-24 and 2021-09-30. Additional sensors included a WET Labs C-Star transmissometer and a WET Labs ECO-AFL fluorometer. Data were connected to the station book of the specific cruise as available in the DSHIP database. Processing of the data including removal of obvious outliers followed the procedures described in CTD Processing Logbook of RV Heincke (hdl:10013/epic.47427). A detailed report on the CTD data of HE585 is available at https://hdl.handle.net/10013/epic.50667c2d-796b-460b-967e-d63d83337aeb.

Beschreibung: Raw data acquired by position sensors on board RV Heincke during expedition HE564 were processed to receive a validated master track which can be used as reference of further expedition data. During HE564 the inertial navigation system IXSEA PHINS III and the GPS receivers Trimble Marine SPS461 and SAAB R5 SUPREME NAV were used as navigation sensors. Data were downloaded from DAVIS SHIP data base (https://dship.awi.de) with a resolution of 1 sec. Processing and evaluation of the data is outlined in the data processing report found at EPIC repository hdl:10013/epic.2f00a207-c487-4f16-8e68-32311d9fc103. Processed data are provided as a master track with 1 sec resolution derived from the position sensors' data selected by priority and a generalized track with a reduced set of the most significant positions of the master track.