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  • Beads  (1)
  • Calcasieu River/Lake  (1)
  • Springer  (2)
  • PANGAEA
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  • Springer  (2)
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  • 1
    ISSN: 1573-5117
    Keywords: heavy metals ; sediments ; Calcasieu River/Lake ; Louisiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The heavy metals Cd, Cr, Cu, Pb, Hg, Ag, and Zn, and the metalloid As were measured in surface sediments at permanent stations located in the Calcasieu River/Lake Complex. The relationships among metal concentrations in different areas of the system were investigated to determine sources, source strength, and transport. The point-source inputs of heavy metals were assumed to be industrial outfalls (Bayou d'Inde) and sewage outfalls (Bayou d'Inde and Contraband Bayou). Although these inputs have not seriously affected the entire river/lake system, stressed regions exist within each bayou. The background levels of arsenic and heavy metals were: 0.60 (As), 0.3 to 1.4 (Cd), 25 (Cr), 10 (Cu), 15 (Pb), 〈 0.05 (Hg), 0.07 (Ag), and 40 mg kg−1 (Zn). Stations near sewage outfalls and industrial outfalls had increased metal concentrations above these background levels, but the increases were restricted to the regions near the outfalls. The metals discharged into the bayous were not being transported to the remainder of the river/lake complex.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 16 (1994), S. 183-188 
    ISSN: 1573-0603
    Keywords: Beads ; Cell culture ; Degradation ; Fibronectin ; Gelatin ; Invasion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is described for culturing invasive cell lines on crosslinked gelatin beads and preparing them for immunocytochemical and morphological observation. Very invasive cells such as Rous sarcoma virus-transformed chicken embryo fibroblasts, and human melanoma LOX and RPMI7951 and breast carcinoma MDA-MB-231 cells will actively degrade this matrix, extending cellular protrusions, called invadopodia, into the sites of degradation. Normal chicken embryo fibroblasts and other non-invasive cell lines do not disrupt the surface of these beads and do not form invadopodia. Invadopodia extending into the bead can be visualized by electron microscopy. Cellular removal of fluorescent fibronectin that has been covalently coupled to the bead surface can be monitored using fluorescence microscopy of frozen-thin-sections. In double label experiments, immunocytochemistry is used to localize antigens in invadopodia at sites of membrane invasion. The materials for bead preparation are inexpensive, and this method has the advantage that many cell types will attach and spread readily on the beads, while only highly invasive cells will invade into the bead.
    Type of Medium: Electronic Resource
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