Publication Date:
2024-03-13
Description:
CARD-FISH was performed as previously described in Ruff et al., (2013; doi:10.1371/journal.pone.0072627) with the following modifications. 4-6 µl of 25-fold diluted sediment were used for filtration. Archaeal cell walls were permeabilized with 0.1M HCl for 2 min to detect ANME-3 cells, or Proteinase K solution (15 µg ml-1 (Merck, Darmstadt, Germany) in 0.05 M EDTA (pH 8), 0.1 M Tris-HCl (pH 8), 0.5 M NaCl) for 2-4 min at room temperature for all other archaea. Bacterial cell walls were permeabilized with lysozyme solution (1000kU/ml) for 60 min at 37°. Cells were stained with DAPI (1µg/ml), embedded in mounting medium and counted in 40-60 independent microscopic fields using an Axiophot II epifluorescence microscope (Carl Zeiss, Jena, Germany).
Keywords:
Anaerobic methanotrophic archaea-3, targeted with ANME-3-1249 oligonucleotide FISH-probe; Archaea, targed with ARCH915 oligonucleotide FISH-probe; ARK-XIX/3b; ARK-XXIV/2; Bacteria, targed with EUB338(I-III) oligonucleotide FISH-probe; Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH); Date/Time of event; Depth, bottom/max; DEPTH, sediment/rock; Depth, top/min; Desulfobulbus, targeted with DBB660 oligonucleotide FISH-probe; Desulfusarcina/Desulfococcus, targeted with DSS658 oligonucleotide FISH-probe; ESONET; European Seafloor Observatory Network; Event label; GC; Gravity corer; Habitat; Håkon Mosby Mud Volcano; HERMIONE; Hotspot Ecosystem Research and Mans Impact On European Seas; Latitude of event; Longitude of event; LOOME; Maria S. Merian; Methylococcaceae, targeted with MTMC701 oligonucleotide FISH-probe; MSM16/2; MSM16/2_809-1; MSM16/2_823-1; MSM16/2_838-1; MSM16/2_847-1; MSM16/2_855-1; MUC; MultiCorer; Multicorer with television; North Atlantic; Norwegian Sea; Polarstern; PS64; PS64/326_PUC-12; PS64/332-1; PS64/336-1; PS64/371-1; PS64/372-1; PS74; PS74/168-1; PS74/169-1_PUC-1; PS74/172-1_PUC-131; PUC; Push corer; Sample code/label; TVMUC
Type:
Dataset
Format:
text/tab-separated-values, 290 data points
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