ISSN:
1040-0397
Keywords:
Dual pulse staircase voltammetry
;
Glucose analysis
;
Fructose analysis
;
Food analysis
;
Sugar analysis
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
A new electroanalytical method, DPSV, employing dual pulses (0.7V and -0.8V) prior to anodic scan (using staircase voltammetry) is developed for simultaneous determination of glucose and fructose. The electrochemistry of both sugars was studied prior to optimizing the operational parameters. The linear ranges were found to be 0.01-2.0 mM for both sugars, precisions (mean = 0.10 mM and n = 8) 1.8% and 2.4% and detection limits (estimated by 3 times the standard deviation) 0.001 and 0.003 mM for glucose and fructose, respectively. To establish the reliability of the method, various food additives were tested and found to give no interference effect under normal concentrations. HPLC method was carried out in parallel with the method for analyzing food, drink and honey samples. No statistically significant difference in the results was obtained for methods.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140070212
Permalink