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  • 1
    Publication Date: 2012-04-24
    Description: The moss Physcomitrella patens is unique among plant models for the high frequency with which targeted transgene insertion occurs via homologous recombination. Transgene integration is believed to utilize existing machinery for the detection and repair of DNA double-strand breaks (DSBs). We undertook targeted knockout of the Physcomitrella genes encoding components of the principal sensor of DNA DSBs, the MRN complex. Loss of function of PpMRE11 or PpRAD50 strongly and specifically inhibited gene targeting, whilst rates of untargeted transgene integration were relatively unaffected. In contrast, disruption of the PpNBS1 gene retained the wild-type capacity to integrate transforming DNA efficiently at homologous loci. Analysis of the kinetics of DNA-DSB repair in wild-type and mutant plants by single-nucleus agarose gel electrophoresis revealed that bleomycin-induced fragmentation of genomic DNA was repaired at approximately equal rates in each genotype, although both the Ppmre11 and Pprad50 mutants exhibited severely restricted growth and development and enhanced sensitivity to UV-B and bleomycin-induced DNA damage, compared with wild-type and Ppnbs1 plants. This implies that while extensive DNA repair can occur in the absence of a functional MRN complex; this is unsupervised in nature and results in the accumulation of deleterious mutations incompatible with normal growth and development.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
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  • 2
    Publication Date: 2009-05-01
    Description: Earthquake rotational effects have been observed for centuries. The first class of rotational seismic models includes two models defined by Mallet (1862) in the mid-nineteenth century based on the rotation of bodies to their underlying structures. These rotational effects satisfactorily explain observed surface rotations. In this short tutorial (based mostly on western literature), we will briefly discuss the historical aspects of earthquake rotational effects.
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
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  • 3
    Publication Date: 2009-05-01
    Description: A new type of rotation seismometer is proposed using a fluid ring-shaped sensor, where the inertial mass is a liquid moving in the tube attached to a pier to measure rotational movements. Rotational components of seismic waves, which are strong enough to twist heavy objects on the Earth's surface in the epicenter zone of an earthquake, decrease rapidly with distance. These weak rotational components may easily vanish in standard seismograms displaying translational displacements. In the proposed fluid, seismometer translational displacements are mutually compensated so that weak rotational vibration components become easily identifiable and recognizable. The technical and physical parameters of such a fluid ring device were tested and evaluated. The proposed fluid seismometer was primarily designed for observing rotational movements in the near field of earthquakes. However, it may also be used for monitoring undesirable rotational effects in large engineering works.
    Print ISSN: 0037-1106
    Electronic ISSN: 1943-3573
    Topics: Geosciences , Physics
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