ISSN:
1432-203X
Keywords:
Auxin
;
Larix decidua Mill
;
Protoplasts
;
Single cell monitoring
;
Somatic embryogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Protoplasts isolated from embryogenic suspension cultures of European larch (Larix decidua Mill.) were cultured in thin alginate layers using a nylon mesh to enable a monitoring of the development of single cells. The patterns of cell division and differentiation are characterized and compared with zygotic embryogenesis to which homologies can only be drawn to some extent when the protoplasts grow in an auxinfree environment. Already at 2.5 μM both 2,4-dichlorophenoxyacetic acid or indole-3-acetic acid cause vacuolation and elongation of individual cells, thus disturbing the process of somatic embryogenesis which generally lacks the precise quantitative patterns occurring in vivo. Prior to the formation of an embryo, a proembryonal mass develops. Oligonucleated products of spontaneous protoplast fusions are able to cellularize even without preceding karyokinesis and perform a normal embryogenic program.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00193728
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