ISSN:
0091-7419
Keywords:
chick embryo fibroblasts
;
cytoskeleton
;
Triton cytoskeleton
;
detergent extraction of chick embryo fibroblasts
;
actin
;
non-muscle
;
LETS protein
;
actin filament bundles
;
intermediate-sized filaments
;
Life Sciences
;
Molecular Cell Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Treatment of chick embryo fibroblasts with 0.5% Triton® X-100 extracts most of the cell protein, leaving an organized part of the cell structure attached to the tissue culture dish. This “Triton cytoskeleton” consists largely of intermediate-sized filaments and bundles of microfilaments. SDS polyacrylamide gel electrophoresis reveals that this cytoskeleton is made up of three main proteins. One protein component is 42,000 daltons and co-migrates with muscle actin. The other two components are 52,000 and 230,000 daltons and remain quantitatively associated with the cytoskeleton during the detergent extraction. The possible identity of these three protein components and their organization into a supramolecular structure is discussed.
Additional Material:
9 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jss.400050203
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