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  • Articles  (7)
  • IAA  (3)
  • autofluorography  (2)
  • Cell culture  (1)
  • Genotypes  (1)
  • Springer  (7)
  • National Academy of Sciences
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (7)
  • Architecture, Civil Engineering, Surveying
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  • Articles  (7)
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  • Springer  (7)
  • National Academy of Sciences
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  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (7)
  • Architecture, Civil Engineering, Surveying
  • 1
    Electronic Resource
    Electronic Resource
    Identification of indole-3-butyric acid as an endogenous constituent of maize kernels and leaves (1989)
    Springer
    Plant growth regulation 8 (1989), S. 215-223 
    Details
    ISSN: 1573-5087
    Keywords: IAA ; IBA ; conjugated IBA ; rooting ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-butyric acid (IBA) was identified by thin layer chromatography, gas-liquid chromatography and gas chromatography-mass spectrometry in kernels and leaves of corn (Zea mays) var. Hazera 224. Free and ester conjugated IBA were present in dry and germinating corn kernels and leaves. This is the first report of IBA in a monocotyledonous plant and, as far as we know, the first evidence for the presence of conjugated IBA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025391
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake and metabolism of indole-3-butyric acid and indole-3-acetic acid by Petunia cell suspension culture (1993)
    Springer
    Plant growth regulation 13 (1993), S. 31-40 
    Details
    ISSN: 1573-5087
    Keywords: autofluorography ; IBA aspartic acid ; IBA glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The uptake and metabolism of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) were studied in suspension cell cultures of Petunia hybrida. The initial uptake of 3H-IBA was much higher than that of 3H-IAA, and after 10 min of incubation with labeled IBA and IAA, 4.6 pM vs 0.35 (39% vs 12% of total applied radioactivity) respectively, were found in the cell extracts. The uptake of IBA reached a plateau of 6.0 pM (62%) after 2 h while that of IAA increased continuously up to 1.5 pM (46%) after 24 h. Following the addition of 40 µM of unlabeled auxin more IBA was taken in initially than IAA (39% vs 12%), but the level almost equalized after 24 h of incubation when IBA uptake reached 890 nM (55%) and IAA 840 nM (46%). IBA was metabolized very rapidly by Petunia cell suspension to new compounds. HPLC of the cell extracts demonstrated a new metabolite after only 2 min of incubation, and after 30 min 60% of the radioactivity was in the new metabolite vs 10% in the IBA. The new compound was resolved by autofluorography to two metabolites but after 24 h only one metabolite was present. The IBA metabolites were identified tentatively as IBA aspartic acid (IBAasp) and IBA glucose (IBAglu). In the medium IBA disappeared at a fast rate and after 24h most of the radioactivity was present in the new metabolite, probably IBAasp. IAA was also converted rapidly to two new metabolites and both were still present after 24 h. No attempt was made to identify the metabolites of IAA. IAA metabolism proceeded at a slower rate, and autofluorography showed that while free IBA disappeared after 0.5 h, free IAA was still present after 1 h of incubation. We postulate that Petunia cells conjugate IBA rapidly to IBAglu which in turn is converted to form IBAasp which probably acts as a ‘slow release’ hormone. Only intact cells were able to metabolize IBA and the reaction was affected by low temperature and anaerobic conditions. The fast rate of IBA uptake, the need for whole cells for the metabolism to proceed, and the fast change of IBA to a new metabolite in the medium, all suggest that both uptake and metabolism of IBA in Petunia cells occur on the cell surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00207589
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  • 3
    Electronic Resource
    Electronic Resource
    Biosynthesis of plant hormones during anaerobic digestion of instant coffee waste (1995)
    Springer
    Plant growth regulation 17 (1995), S. 127-132 
    Details
    ISSN: 1573-5087
    Keywords: anaerobic methanogenic thermophilic digestion ; auxin ; biogas ; IAA ; IBA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A large amount of solid waste remains after the production of instant coffee. This waste has to be moved to dumps, where it poses a threat of environmental pollution. Treatment of this waste by anaerobic methanogenic thermophilic digestion produced, besides biogas, a digested slurry which was used as a growth medium for horticulture, and proved to be a suitable and economical substitute for peat moss. Biological tests with mung bean cuttings and Grevillea plantlets showed promotional effects on rooting of the slurry and its sieved fraction extract, washed with water (Capul). Green coffee beans, instant coffee waste, its anaerobically-digested slurry and Capul were extracted by various methods and the extracts were analyzed by TLC, HPLC and GC/MS. Examinations showed clearly the presence of IAA and IBA in free and bound forms in all the substrates. The values of free and bound IAA were calculated by use of an internal standard and GC/MS. The amount of conjugated IAA was found to be much higher than that of free IAA, in both the coffee beans and instant coffee waste (11.1 vs 2.7 nmol g−1, respectively). In the digested slurry and Capul, however, most of the IAA was present as the free form and was approximately 23.5–33.0 nmol g−1, which is almost ten times more than in the waste, and almost twice the total amount of IAA in coffee beans. It is postulated that the high levels of free IAA in the digested instant coffee waste are a result of catabolism of tryptophan by anaerobic bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024172
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  • 4
    Electronic Resource
    Electronic Resource
    Indole-3-butyric acid in Arabidopsis thaliana (1993)
    Springer
    Plant growth regulation 13 (1993), S. 189-195 
    Details
    ISSN: 1573-5087
    Keywords: Arabidopsis thaliana ; autofluorography ; auxin conjugates ; IBA-glucose synthase ; indole-3-butyric acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Indole-3-butyric acid (IBA) was metabolized by seedlings of Arabidopsis thaliana cultivated in liquid medium under sterile conditions to two major metabolites. One metabolite was hydrolyzed by 1 N NaOH and β-glucosidase and was tentatively identified as IBA-glucose and the other was hydrolyzed by 7 N NaOH and amidase and was identified as an amide-linked conjugate. IBA-glucose synthase activity was found in a soluble enzyme fraction after incubation of 3H-IBA, IBA and UDP-glucose. The labelled reaction product had an Rf value comparable to IBA glucose and stained positive with Ehmann reagent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024261
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  • 5
    Electronic Resource
    Electronic Resource
    Advances in salt tolerance (1987)
    Springer
    Plant and soil 99 (1987), S. 17-29 
    Details
    ISSN: 1573-5036
    Keywords: Cell culture ; Molecular biology ; Plant breeding ; Salinity ; Seawater ; Selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Advances in and prospects for the development of salt tolerant crops are discussed. The genetic approach to the salinity problem is fairly new, but research has become quite active in a short span of time. Difficulties and opportunities are outlined. Salinity varies spatially, temporally, qualitatively, and quantitatively. In addition, the responses of plants to salt stress vary during their life cycle. Selection and breeding, including the use of wide crosses, are considered the best short-term approaches to the development of salt tolerant crops, but the new biotechnological and molecular biological techniques will make increasingly important contributions. Cooperation is called for among soil and water scientists, agronomists, plant physiologists and biochemists, cytologists, and plant geneticists, breeders, and biotechnologists. Given such cooperation and adequate support for these endeavors, the potential for increasing productivity in salt-affected areas can be realized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02370150
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of ethylene treatment on transport and metabolism of indole-3-butyric acid in citrus leaf midribs (1992)
    Springer
    Plant growth regulation 11 (1992), S. 357-362 
    Details
    ISSN: 1573-5087
    Keywords: Citrus ; conjugation ; ethylene ; IAA ; IBA ; polar transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transport and metabolism of radiolabeled indole-3-butyric acid (IBA) were studied in midrib sections of Cleopatra mandarin (Citrus reticulata Blanco) and compared to that of indole-3-acetic acid (IAA). Exogenous IBA was metabolized by the midribs to a polar compound, probably an ester conjugate. Ethylene pretreatment of the midribs reduced their capacity to metabolize IBA by ca. 70% as compared to air pretreatment. IBA transport capacity in the leaf midribs was ca. two times greater in the basipetal direction than the acropetal. The basipetal transport capacity of 3H-IBA was lower than that of 14C-IAA (ca. 24% and 39% of the uptake, respectively). While ethylene treatment reduced basipetal transport of IAA by ca. 70% it did not affect the transport of IBA. Most of the transported label was found as free IBA, but the reduction of IBA conjugation by ethylene treatment did not affect the transport capacity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00130642
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  • 7
    Electronic Resource
    Electronic Resource
    Salt-tolerant crops: origins, development, and prospects of the concept (1985)
    Springer
    Plant and soil 89 (1985), S. 187-198 
    Details
    ISSN: 1573-5036
    Keywords: Breeding ; Genotypes ; Halophytes ; Saline water ; Salt-affected soils ; Selection ; Stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic approach to the problems posed by salt-affected soils and water,i.e., breeding crops resistant to salinity stress, is traced to two principal origins: the European ecological interest in halophytes, and the exigencies of growing crops in the arid and semi-arid lands of the American West. The point is made that breeding for resistance to salinity stress cannot be divorced from breeding for various other desirable traits of mineral plant nutrition and metabolism. A survey is conducted of the existing body of information on breeding for desiderata of mineral nutrition in general and salt tolerance in particular. The prospects of breeding crops for salt tolerance are discussed, with emphasis on a) its relation to breeding for resistance to other mineral stresses; b) field trials; c) collaboration between plant physiologists and geneticist-breeders; and d) extensive exploration of germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182242
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