ALBERT

Description: The cellular delivery of cell-impermeable and water-insoluble molecules remains an ongoing challenge to overcome. Previously, we reported amphipathic cyclic peptides c[WR]4 and c[WR]5 consisting of alternate arginine and tryptophan residues as nuclear-targeting molecular transporters. These peptides contain an optimal balance of positive charge and hydrophobicity, which is required for interactions with the phospholipid bilayer to facilitate their application as a drug delivery system. To further optimize them, we synthesized and evaluated a multivalent tricyclic peptide as an efficient molecular transporter. The monomeric cyclic peptide building blocks were synthesized using Fmoc/tBu solid-phase chemistry and cyclization in the solution and conjugated with each other through an amide bond to afford the tricyclic peptide, which demonstrated modest antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae, Pseudomonas aeruginosa, and Escherichia coli (E. coli) with a minimum inhibitory concentration (MIC) of 64–128 µg/mL. The tricyclic peptide was found to be nontoxic up to 30 µM in the breast cancer cell lines (MDA-MB-231). The presence of tricyclic peptide enhanced cellular uptakes of fluorescently-labeled phosphopeptide (F’-GpYEEI, 18-fold), anti-HIV drugs (lamivudine (F’-3TC), emtricitabine (F’-FTC), and stavudine (F’-d4T), 1.7–12-fold), and siRNA (3.3-fold) in the MDA-MB-231 cell lines.

Description: Dissolved oxygen (DO) is an important parameter to monitor in processes applicable in, for example, water technology and fermentation. In this study, we report the manufacturing of a membrane-less amperometric sensor that is based on the electrocatalytic reduction of oxygen. The sensor was tested in pH-neutral KNO3 solutions and in a microbial fermentation to monitor the consumption of dissolved oxygen. The nitrogen and boron-doped reduced graphene oxide (N,B-HRGO) is used as an electrocatalyst for oxygen reduction. One step co-doping of nitrogen and boron on graphene oxide is performed using the hydrothermal method. The sensor responded linearly to the DO concentration. A sensitivity of 0.2 µA/mg·L−1 O2 is obtained for the DO concentration of 1.5 to 10 mg·L−1 O2. The membrane-less N,B-HRGO based DO sensor is successfully tested in an Amycolotopsis methanolica fermentation by monitoring the dissolved oxygen in real-time. The sensor detected the consumption of oxygen during the growth of A. methanolica, which shows the compatibility of N,B-HRGO as electrode material for amperometric measurement of dissolved oxygen in aerobic fermentation. This membrane-less amperometric sensor can be used to fabricate microdevices for microbioreactor applications.

Description: This paper presents a compact 1 × 4 antipodal Vivaldi antenna (AVA) array for 5G millimeter-wave applications. The designed antenna operates over 24.19 GHz–29.15 GHz and 30.28 GHz–40.47 GHz frequency ranges. The proposed antenna provides a high gain of 8 dBi to 13.2 dBi and the highest gain is obtained at 40.3 GHz. The proposed antenna operates on frequency range-2 (FR2) and covers n257, n258, n260, and n261 frequency bands of 5G communication. The corrugations and RT/Duroid 5880 substrate are used to reduce the antenna size to 24 mm × 28.8 mm × 0.254 mm, which makes the antenna highly compact. Furthermore, the corrugations play an important role in the front-to-back ratio improvement, which further enhances the gain of the antenna. The corporate feeding is optimized meticulously to obtain an enhanced bandwidth and narrow beamwidth. The radiation pattern does not vary over the desired operating frequency range. In addition, the experimental results of the fabricated antenna coincide with the simulated results. The presented antenna design shows a substantial improvement in size, gain, and bandwidth when compared to what has been reported for an AVA with nearly the same size, which makes the proposed antenna one of the best candidates for application in devices that operate in the millimeter frequency range.

Description: Millets are an underutilized and important drought-resistant crop, which are mainly used for animal feed. The major constituent in millet is starch (70%); millet starch represents an alternative source of starches like maize, rice, potato, etc. This encouraged us to isolate and characterize the starches from different millet sources and to evaluate the application of these starches in edible film preparation. In the present study, the physicochemical, morphological, and film-forming characteristics of millet starches were studied. The amylose content, swelling power, and solubility of millet starches ranged from 11.01% to 16.61%, 14.43 to 18.83 g/g, and 15.2% to 25.9%, respectively. Significant differences (p 〈 0.05) were found with different pasting parameters, and the highest peak (2985 cP), breakdown (1618 cP), and final viscosity (3665 cP) were observed for barnyard, proso, and finger millet starch, respectively. Little millet starch achieved the highest pasting temperature. All starches showed A-type crystalline patterns, and relative crystallinity was observed at levels of 24.73% to 32.62%, with proso millet starch achieving the highest value. The light transmittance of starches varied from 3.3% to 5.2%, with proso millet starch showing the highest transparency. Significant differences (p 〈 0.05) were observed in the water solubility, thickness, opacity and mechanical characteristics of films. The results of the present study facilitate a better assessment of the functional characteristics of millet starches for their possible applications in the preparation of starch films.