ALBERT

Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was determined some important extracted materials, Such as : Alginates of sodium & calcium and alginic acid in three species of brown seaweeds (Sargassum illicifolium , Cystoseira indica and Nizimuddinia zanardinii) during in different season in 1387 in coastal erea of Sistan and Baluochestan Provience. At First seaweeds sample were colected from sea beach and transferd to lab, so washed in fresh water and dried in the sun. So were under Alkaloid extraction with sodium carbonate (5 Persent), that were obtined fluid after filteration. The sodium alginate fibers extracted from fluid were combined by Alcohol. The calacium alginate fibers were deposited by calacium chlorids (5%) and Alginic acid resulted with chloridric acid (1N). The extraction yield of sodium alginate, calacium alginate and alginic acid in different samples of seaweeds with following Explaination: The presentage we have got, can be said , the present average of sodium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 28.4±2 , 28.9±2 , 27.2±1.9 present, the present average of sodium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 19.7±1.4, 18.6±1.4, 19.2±1.3 present and the present average of sodium alginate at Nizimuddinia in Spring were 23.4±1.5, 23.8±1.5 , 20.6±1.5 in these three areas. The present average of calacium alginate at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 33.7±0.5, 33.7±0.8, 33.1±1 present, the present average of calacium alginate at Cystoseira in Spring in three areas at above mentioned goes in this way: 28.8±1.5, 27.4±1, 27.4±1.5 present and the present average of calacium alginate at Nizimuddinia in Spring were 35.9±2.8, 23.9±1.5 , 35.9±2.8 in these three areas. The present average of Alginic acid at Sargassum in Spring season in three areas (Chabahar, Tang and Pasabandar ): 19.2±1.6 , 25.6±1.7 , 18.4±1.1 present, the present average of Alginic acid at Cystoseira in Spring in three areas at above mentioned goes in this way: 16.8±1.2, 15.8±1.1, 16.4±1 present and the present average of Alginic acid at Nizimuddinia in Spring were 19.2±1.6, 19.6±1.7 , 18.4±1.5 in these three areas. The present average of sodium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar ) Respectively: 20.8±1, 21±1, 27.8±1.9 present, the present average of sodium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 13.2±1, 14.1±0.7 present and the present average of sodium alginate at Nizimuddinia in Summer were 25.3±2.3, 16.7±2.1, 15.3±1.3 in these three areas. The present average of calacium alginate at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar): 29.5±2.1 , 29.7±2 , 28.3±2.2 present, the present average of calacium alginate at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 20.2±0.9, 20±0.7 present and the present average of calacium alginate at Nizimuddinia in Summer were 25.3±2.3, 29.7±2 , 23.1±1.5 in these three areas. The present average of Alginic acid at Sargassum in Summer season in three areas (Chabahar, Tang and Pasabandar): 19.5±0.7 , 19.7±0.6 , 18.7±0.7 present, the present average of Alginic acid at Cystoseira in Summer in three areas at above mentioned goes in this way: 21±1.1, 11.5±1.4, 11.1±1.5 present and the present average of Alginic acid at Nizimuddinia in Summer were 14.8±1.3, 15.3±1.3 , 14.6±1 in these three areas. The present average of sodium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar) Respectively: 31.5±4.3, 31.6±4.8, 29.4±4.8 present, the present average of sodium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 23±2.7, 21.4±2.8, 21.4±2.7 present and the present average of sodium alginate at Nizimuddinia in Autumn were 25.8±2.2, 26.4±2.1 , 23.4±1.9 in these three areas. The present average of calacium alginate at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar): 43.3±1.6, 42.8±2.7, 41.7±2.9 present, the present average of calacium alginate at Cystoseira in Autumn in three areas at above mentioned goes in this way: 34.3±5.9, 32.4±5.5, 30.9±5.2 present and the present average of calacium alginate at Nizimuddinia in Autumn were 50±7.1, 50.2±7.5, 45.6±5.2 in these three areas. The present average of Alginic acid at Sargassum in Autumn season in three areas (Chabahar, Tang and Pasabandar): 28±3.8, 28±3.5, 26±3.9 present, the present average of Alginic acid at Cystoseira in Autumn in three areas at above mentioned goes in this way: 20.2±1.8, 19.2±2.1, 18.7±1.6 present and the present average of Alginic acid at Nizimuddinia in Autumn were 25.9±3, 26.1±2.9, 24.6±3.6 in these three areas. The present average of sodium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar) Respectively: 35±1.6, 35.2±1.8 , 33.8±1.9 present, the present average of sodium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 24.1±2, 22.9±1.2, 23.3±0.7 present and the present average of sodium alginate at Nizimuddinia in Winter were 28.2±1.3, 29.2±1.7, 26.2±1.8 in these three areas. The present average of calacium alginate at Sargassum in Winter season in three areas (Chabahar, Tang and Pasabandar): 45.6±1.9, 45±2, 44.6±2.5 present, the present average of calacium alginate at Cystoseira in Winter in three areas at above mentioned goes in this way: 38.2±2.8, 35.9±3.4, 35.6±3.5 present and the present average of calacium alginate at Nizimuddinia in Winter were 56.4±3.5, 56±3.5, 51.8±2.5 in these three areas. The present average of Alginic acid at Sargassum in winter season in three areas (Chabahar, Tang and Pasabandar): 31.8±1.2, 32.1±0.9, 30.9±1.2 present, the present average of Alginic acid at Cystoseira in Winter in three areas at above mentioned goes in this way: 21.1±1.2, 20.4±1.4, 19.3±1.4 present and the present average of Alginic acid at Nizimuddinia in Winter were 26.4±2.1, 27±2.2, 24.6±2.2 in these three areas. The more amount of calacium alginate in Autumn can be found at Nizimuddinia in Tang area , wite amount of 50.2 present and the lates amount of Alginic acid at Cystoseira can be found in Summer with 11.1 present. If the Alginic acid become more than 20 present, it can be economical, that it can be showed more than 20 present in Autumn and Winter. According to this results, One way ANOVA showed that average of Alginates were not similar and significant differences ( P〈0.05 ) between species.

Description: In this project, the nutritional effects of Sargassum illicifolium Chabahar bay-Oman Sea, on growth and survival rates of whiteleg shrimp (Litopenaeus vannamei) were studied. The seaweed collected from 6 coastal area, rinsed, dried, powdered and measured the nutritional values in laboratory for surveying statistically. According to the high nutritional value of Tis coastal seaweed, this variate seaweed powder, replaced with protein resources (fish meal and Soy and Wheat) of whiteleg shrimp feed which was formulated by Havorash feed factory of Boshehr in four treatments (A: as control without any replacement) B: with 5%, C: 10 % and D: 15% seaweed replacement, each with three replicates in order to obtain isonitrogenus 33% CP., and Isocaloric (13% fat and 15% carbohydrate) feed. The weighed milled ingredients were carefully mixed using a laboratory food mixer. The mixtures were primed with 30% hot water to yield a suitable pulp. Wet diets were made into 2 mm pellet size and dried at 40 °C in a drying cabinet and maintained in standard condition which was used according to daily need shrimp, calculated by each 10 days biometry. Water stability and absorbtion capacity of the pellets in sea water were measured and compared statistically. Juvenile shrimps (Initial body weight =3 g) brought from Jask hatchery, acclimazed for one week in Chabahar hatchery condition and feeding daily 3-5% body weight. Abiotical parameters and weight and length biometrics were measured two days and 10 days, respectively. After 45 days and final biometry, FCR, CF, SGR, caracas analysis, muscle colourimetery with HPLC were done, tasted with pp Plot for determining the parametric data and statistically differences using one – way ANOVA, Duncan test of SPSS software. The Tis coastal seaweed with 9.8% CP, 2% lipid and 23% carbohydrate had higher nutritional value compared to the other gathered seaweed. Also amino acid and fatty acid profiles, vitamins and minerals were measured in all seaweed samples each, with three replications. As result, the water stability of D feed treatment in seawater (98%) and C (97%) had statistical differences with A and B (95% stability) (P〈0.05). Water absorption capacity of feeds after one hour immersion in seawater showed significance difference between D (110%) and three others, C(100%), B(85% and control(80%) (P〈0.05). Shrimp growth data, after the end of experiment revealed that seaweed feed treatments had no any differences with control group significantly. However, the absolute growth rate datas of D treatment were higher than others and the lower weight and lenght were measured in control group shrimp. FCR had difference between seaweed treatments and control statistically. There are no any differences between caracas lipid treatments (P〉0.05) but Cholestrol content of, showed differences between all, significantly (P〈0.05) which was the highest (121.68±12.12) in D and the lowest in A (147.92±11.02). Feed treatment D and C performed colour changes pink partial orang and pink in shrimp muscle with no any difference compared to white and none colour in shrimp were fed B and A feed treatments . It seems this colour changing can be playing a major role in market acceptability.

Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.

Description: A sharp decline in natural population of Caspian brown trout lead to implementing of restocking program in Iran. Along the stock recovery program, the artificial production of this species has been attracted too much attention in recent years. The objective of the study is to assess the effects of protein and energy levels of diet on fish performance, growth efficiency of Caspian brown trout in order to develop a proper diet for production of this species. Six experimental diets were formulated by replacing casein as a pure protein source with fat as an energy source in the diets according to a 3×2 factorial design. The diet was assigned to 18 tanks with 50 fish each, with three replicated for each diet. The experiment was conducted for 8-weeks with Caspian brown trout with an initial body weight of around 7 g. Caspian brown trout demonstrated a better FCR and a larger SGR at lower protein levels (45 and 50%) in comparison to high protein level (55%; P〈0.05). Protein efficiency ratio improved by a decrease in protein content of the diet (P〈0.05), but energy content of diets does not affect any growth related parameter such as FCR, SGR and PER. There was also no interaction between protein and energy levels in the growth related parameters, suggesting the effect of protein on the growth parameters in Caspian brown trout did not depend on energy levels of diet. In conclusion diet containing 50% protein can support the maximum growth. High dietary energy level of the diet dose in cooperate in protein sparing effect.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This survey was designed in the Helleh region, to understand effects of aquaculture industry on coastal waters of Busher, in 2000. Sediment and water samples were collected from effluent canal, influent canal and sea during the 7 month period (harvest and post harvest season). The variations of selected water quality parameters were controlled monthly. Results show that, in effluent canal, estimated parameters of water was higher than other stations every month, and tend to decreased after being discharged into the sea and got closed to normal level. Comparison of above data with permit able range of municipal and aquaculture waste and comparison of present data with data of normal condition of region, show that, aquaculture industry of Helleh region has not had any obvious negative effect on the coastal waters of Bushehr, in the culture year of 2000.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Considering the importance of rainbow trout (Oncorhynchus mykiss) in supplying required protein of people and effort to increase the efficiency of these fish reproduction, some related factors such as sperm quality and potential fertility of male are necessary. The aim of this study was to find out the effects of different dosages of Arginine on the biochemical parameters (including LDH, AST, ALT, ALP, iron, magnesium, phosphorous, chloride, calcium, sodium, Potassium, cholesterol, uric acid, urea, fructose, glucose, total protein and pH) of rainbow trout seminal plasma. For this purpose, five practical diets (each consisting of 3 triplicates) were supplemented with Arginine at 0.00 (Control), 0.50, 1.00, 1.50 and 2.00%. Broodstock feed last for 90 days. At the end of the feeding period one fish was captured from each replicate in order to collect their semen. Results indicate that there were no significant differences in LDH, ALP, Fe^2+ and P content among different treatments. The lowest level of AST and ALT and the highest level of Ca^2+ and Mg^2+ ions were obsereved in the treatment fed with 1.50% of Arginine which showed significant differences with other treatments (p〈0.05). Moreover, the amount of Cl- , Na^+ and K^+ ions were significantly increased in the seminal plasma in fish which were fed on diets containing arginine in comparison to control. As the amount of Arginine were increased, the levels of uric acid stepped up significantly in contrast to the urea and glucose. The highest amounts of cholesterol, fructose and total protein were observed in the treatments fed on 2.00, 0.50 and 1.00% of Arginine, respectively, that showed significant differences with other treatments (p〈0.05). The highest pH value was assayed in the 1.50% of Arginine treatment. The results of the Pearson correlation coefficient showed a significant positive correlation between Ca^2+ and Mg^2+ ions and Na^+ with K^+ and Clions (r=0.750, r=0.769 and r= 0.938, p〈0.01), respectively. On the other hand, a significant negative correlation between cholesterol with Cl- , Na^+ , K^+ , ALT and LDH (r=- 0.764, r=-0.724 and r=-0.728, p〈0.01) and (r=-0.531 and r=-0.560, p〈0.05) and also a significant positive correlation between K^+ and Clions (r=0.836, p〈0.01) were observed. Finally, it can be expressed that the levels of most of the ions were increased and there was a reduction in the levels of enzymes in seminal plasma of fish which were fed with practical diet including 1.5% of Arginine. So it can be recommended that adding this value of Arginine to the diets of rainbow trout broodstock, would improve the sperm quality which results in the enhancement of efficiency in rainbow trout reproduction.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: Due to lack of access to comprehensive and up-to-date information regarding equipment, personnel, laboratory services tariffs and chemicals in the laboratories of Iran Fisheries Research Institute and felt the necessity of having this information for optimal management of the laboratories, it was decided with regard to all information needs in this area and using previous experiences in this regard as well as the experiences of other institutions and related organizations at the national (and even international) level, the system would be designed. The main purpose of implementing this plan was to establish and implement a software system based on web technology to create a comprehensive database of the Fisheries Research Institute's laboratories, so that in addition to organizing information, using periodic reports on various related areas a more appropriate monitoring and management system could be applied. Fortunately, after the design and implementation stages, the system in early January 2011 was fully applicable. The main parts of this system include portal; database; advanced data entry, search and reporting capabilities; direct tests order system and discussion forums.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to codify the guideline and indexes of optimal management in warm-water fish farms to attain sustainable aquaculture which they are population density, species diversity, brood stock'smanagements, , capacity determination,growth and harvesting managements in cultural ponds. As results, these guideline and indexes were codifies for these fish farms and propagation centers.It's obviously that these sustainable patterns can be used for improving of permanent development for this aquacultural industry.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: The objective of this study was to evaluate 4 levels of feeding defined as 1.ad libitum to satiation, 2.Standard feeding (based on fish size and water temperature), 3.10% less than that of standard feeding and 4.10% more than that of standard feeding on growth, feed conversion rate (FCR) and carcass quality of rainbow trout (Oncorhynchus mykiss) reared in 12 aerated flow-through fiber glass tanks (2500l rearing volume, n=150 fish per tank, body weight average 45 g) comprised of 3 replicates under the condition of brackish water (salinity 8.4 g/l) in Bafgh Fisheries Research Station in center of Iran. The increase in average body weight (BW) and size of fish in different feeding groups was significantly different (P〈0.01) during the experiment (101 days) and the highest average BW and size increase occurred in the group fed ad libitum to satiation. On the contrary, FCR (average±SE: 1.27±0.09) in this group was statistically lower than that of the other groups (P〈0.01). The chemical composition of carcass was significantly influenced by the level of feeding (P〈0.01) and the percent of moisture, crude protein and ash was the lowest in the group fed ad libitum to satiation while the crude fat percent in this group was the highest of all. According to the results obtained in this study, an ad libitum feeding regime is suggested for rainbow trout production in fish culture systems of central desert regions in Iran under the condition of brackish water. However, further investigations are needed to draw firm conclusions, especially in earth pond cultures with different conditions, enriched in live food.

Description: Iranian Fisheries Science Research Institute

Description: Published