ISSN:
1432-2242
Keywords:
Cytoplasmic male-sterility
;
Sorghum bicolor
;
Mitochondrial DNA clones
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have demonstrated that sorghum DNA sequences of mitochondrial origin can be used to distinguish different male-sterility-inducing cytoplasms. Six DNA clones containing single-copy mitochondrial sequences were hybridized on Southern blots to restriction enzyme-digested DNA of 28 sorghum lines representing sources of different cytoplasmic male-sterility (CMS) groups. Four cytoplasmic types were defined on the basis of the pattern of DNA fragments detected. Similar analyses of 50 additional diverse sorghum accessions suggested that three of the four cytoplasmic types may be diagnostic for CMS. Also, three other cytoplasmic types were discovered. These and other mitochondrial DNA clones may be useful molecular tools for “fingerprinting” sterility-inducing cytoplasms in breeding programs, determining cytoplasmic diversity among germ plasm accessions, and identifying new sources of cytoplasm that induce male sterility.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00222941
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