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  • Articles  (2)
  • Polyamine  (1)
  • mechanical error  (1)
  • optimization  (1)
  • Springer  (2)
  • Institute of Physics (IOP)
  • 1
    ISSN: 1432-2048
    Keywords: Arginine decarboxylase ; Auxin in somatic embryogenesis ; Cell culture (polyamines) ; Dnucus (polyamines in embryogenesis) ; Embryogenesis somatic ; Polyamine ; S-Adenosylmethionine decarboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyamine levels and the activities of two polyamine biosynthetic enzymes, arginine decarboxylase (EC 4.1.1.19) and S-adenosylmethionine decarboxylase (EC 4.1.1.50), were determined during somatic embryogenesis of carrot (Daucus carota L.) cell cultures. Embryogenic cultures showed severalfold increases in polyamine levels over nondifferentiating controls. A mutant cell line that failed to form embryos but grew at the same rate as the wild-type line also failed to show increases in polyamine levels, thus providing evidence that this increased polyamine content was in fact associated with the development of embryos. Furthermore, inhibition of these increases in polyamines caused by drugs inhibited embryogenesis and the effect was reversible with spermidine. The activities of arginine decarboxylase and Sadenosylmethionine decarboxylase were found to be suppressed by auxin; however, the specific effects differed between exogenous 2,4-dichlorophenoxyacetic acid and endogenous indole-3-acetic acid. The results indicate that increased polyamine levels are required for cellular differentiation and development occurring during somatic embryogenesis in carrot cell cultures.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Multibody system dynamics 2 (1998), S. 145-168 
    ISSN: 1573-272X
    Keywords: tolerance ; clearance ; optimization ; mechanical error ; mechanism ; lubricated joint
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper addresses an analytical approach to tolerance optimization for planar mechanisms with lubricated joints based on mechanical error analysis. The mobility method is applied to consider the lubrication effects at joints and planar mechanisms are stochastically defined by using the clearance vector model for mechanical error analysis. The uncertainties considered in the analysis are tolerances on link lengths and radial clearances and these are selected as design variables. To show the validity of the proposed method for mechanical error analysis, it is applied to two examples, and the results obtained are compared with those of Monte Carlo simulations. Based on the mechanical error analysis, tolerance optimizations are applied to the examples.
    Type of Medium: Electronic Resource
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