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  • elicitor  (2)
  • Springer  (2)
  • Institute of Physics
  • 1
    Electronic Resource
    Electronic Resource
    Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato (1993)
    Springer
    Plant molecular biology 22 (1993), S. 279-291 
    Details
    ISSN: 1573-5028
    Keywords: wounding ; elicitor ; plant defense ; gene expression ; tuber ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene consisting of 1015 bp of 5′-flanking sequence and part of the first exon of STH-2 fused to the bacterial β-glucuronidase gene was highly-expressed in tubers of transgenic potato plants after wounding and elicitor treatments. The levels of activity observed in these transgenic plants parallel those observed for the accumulation of STH-2 mRNAs under similar conditions. This indicates that cis-acting elements necessary for the proper activation of the gene are present within 1 kb of 5′-flanking sequences. Functional analysis of 5′ deletions of the STH-2/GUS constructs by transient expression in leaf protoplasts revealed the presence of an upstream regulatory sequence between -135 and -52 which contains a TGAC motif, and a possible negative regulatory region between -52 and -28. A factor present in nuclear extracts of wounded potato tubers was found to bind specifically to nucleotides located between -135 to -105, suggesting that this region contains important cis-regulatory elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014935
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  • 2
    Electronic Resource
    Electronic Resource
    Differential accumulation of potato tuber mRNAs during the hypersensitive response induced by arachidonic acid elicitor (1987)
    Springer
    Plant molecular biology 9 (1987), S. 335-342 
    Details
    ISSN: 1573-5028
    Keywords: potato tuber ; elicitor ; arachidonic acid ; cDNA cloning ; gene expression ; PR proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Accumulation of messenger RNAs in potato tuber discs was analysed during the hypersensitive response induced by treatment with the biotic elicitor arachidonic acid. In vitro translation of polysomal poly(A)+ RNAs indicated that the accumulation of some sixteen mRNAs varied following treatment with arachidonic acid, and that the level of thirteen of these was increased. Two cDNA closes (pSTH-1 and-2) were isolated from a library of elicitor-treated tissue cDNAs. Northern blot analysis using these clones as molecular probes indicated that the levels of at least two mRNAs were markedly increased after elicitor treatment. In hybrid-released translation experiments, each of the cDNA clones selected more than one mRNA. Translation of these mRNAs yielded two polypeptides of Mr 45 000 (for the pSTH-1 clone), and three polypeptides of Me 17 000 (for the pSTH-2 clone). The low molecular weight polypeptides may correspond to potato pathogenesis-related (PR) proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014908
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    Paper (German National Licenses)
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