ISSN:
1573-5028
Keywords:
Nicotiana alata
;
repetitive DNA
;
self-incompatibility
;
S-RNase gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Genomic clones encoding the S 2- and S 6-RNases of Nicotiana alata Link and Otto, which are the allelic stylar products of the self-incompatibility (S) locus, were isolated and sequenced. Analysis of genomic DNA by pulsed-field gel electrophoresis and Southern blotting indicates the presence of only a single S-RNase gene in the N. alata genome. The sequences of the open-reading frames in the genomic and corresponding cDNA clones were identical. The organization of the genes was similar to that of other S-RNase genes from solanaceous plants. No sequence similarity was found between the DNA flanking the S 2- and S 6-RNase genes, despite extensive similarities between the coding regions. The DNA flanking the S 6-RNase gene contained sequences that were moderately abundant in the genome. These repeat sequences are also present in other members of the Nicotianae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00042070
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