Publication Date:
2022-05-25
Description:
Author Posting. © Author(s), 2012. This article is posted here by permission of Informa Healthcare USA, for personal use, not for redistribution. The definitive version was published in BioTechniques 52 (2012): 39-43, doi:10.2144/000113802.
Description:
The selection, isolation, and accurate positioning of single cells in three dimensions are increasingly desirable in many areas of cell biology and tissue engineering. We describe the application of a simple and low cost dielectrophoretic device for picking out and relocating single target cells. The device consists of a single metal electrode and an AC signal generator. It does not require microfabrication technologies or sophisticated electronics. The dielectrophoretic manipulator also discriminates between live and dead cells and is capable of redistributing intracellular organelles.
Description:
This research was funded by NIH-NCRR grant P41 RR001395 and supported by The Eugene and Millicent Bell Fellowship Fund in Tissue Engineering, the Hermann Foundation Research Development Fund Award, the Dennis and Alix Robinson Memorial, and the MBL Bell Center, grant GM092374.
Description:
2012-07-01
Keywords:
Cell sorting
;
Cell isolation
;
Cell manipulation
;
Organelle manipulation
;
Dielectrophoresis
Repository Name:
Woods Hole Open Access Server
Type:
Article
Format:
application/pdf
Format:
video/wmv
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