Publication Date:
2018-01-01
Description:
Watermelons(Citrullus lanatus)are known to have sufficient amino acid content. In this study, watermelons grown and consumed in Malaysia were investigated for their amino acid content, L-citrulline and L-arginine, by the isocratic RP-HPLC method. Flesh and rind watermelons were juiced, and freeze-dried samples were used for separation and quantification of L-citrulline and L-arginine. Three different mobile phases, 0.7% H3P04, 0.1% H3P04, and 0.7% H3P04 : ACN (90 : 10), were tested on two different columns using Zorbax Eclipse XDB-C18and Gemini C18with a flow rate of 0.5 mL/min and a detection wavelength at 195 nm. Efficient separation with reproducible resolution of L-citrulline and L-arginine was achieved using 0.1% H3P04on the Gemini C18column. The method was validated and good linearity of L-citrulline and L-arginine was obtained withR2= 0.9956,y=0.1664x+2.4142andR2=0.9912,y=0.4100x+3.4850, respectively. L-citrulline content showed the highest concentration in red watermelon of flesh and rind juice extract (43.81 mg/g and 45.02 mg/g), whereas L-arginine concentration was lower than L-citrulline, ranging from 3.39 to 11.14 mg/g. The isocratic RP-HPLC method with 0.1% H3P04on the Gemini C18column proved to be efficient for separation and quantification of L-citrulline and L-arginine in watermelons.
Print ISSN:
1687-8760
Electronic ISSN:
1687-8779
Topics:
Chemistry and Pharmacology
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