Publication Date:
2013-01-01
Description:
A putative protease gene (aprE) from the thermophilic bacteriumCoprothermobacter proteolyticuswas cloned and expressed inBacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8–10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, theC. proteolyticusprotease has potential applications in industries such as the detergent market.
Print ISSN:
2356-6140
Electronic ISSN:
1537-744X
Topics:
Natural Sciences in General
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