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  • 1
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    Structure-Immunogenicity Relationship of α- and β-Tetrasaccharide Glycoforms from Bacillus anthracis Exosporium and Fragments Thereof (2018)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2018-08-20
    Description: The tetrasaccharide (2-O-methyl-4-(3-hydroxy-3-methylbutamido)-4,6-dideoxy-α-d-glucopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→3)-α-l-rhamnopyranosyl-(1→2)-l-rhamnopyranose) from the major exosporium protein (BclA) of Bacillus anthracis has been proposed as a target for development of diagnostics and immune therapy or prophylaxis. While the immunodominant character of the anthrose residue has been previously elucidated, the role of the stereochemical configuration of the downstream rhamnose is unknown. Because the linkage of this residue to the GlcNAc bridging the glycan and the protein is lost during isolation of the tetrasaccharide, its α- and β-glycoforms have been synthesized. Herein, we prepared neoglycoconjugates from a series of fragments of the tetrasaccharide, including the complete α- and β-tetrasaccharide glycoforms, a 2-demethoxylated version of the α-tetrasaccharide, and the α- and β-trirhamnosides and CRM197. By immunization of mice, we showed that the anti α- and β-tetrasaccharide serum equally recognized both glycoforms. In contrast the sera produced following immunization with the α- and β-trirhamnoside fragments exhibited higher recognition for their own antigens than for their anomeric counterparts. The anti α- and β-tetrasaccharide sera recognized Sterne spores in a comparable fashion. ΔBclA spores not expressing the major exosporium protein were also recognized by the same sera, while mutants that produced the carbohydrate antigen with deletion of either rhamnose or anthrose were not. The tetrasaccharide could, therefore, be expressed in proteins other than BlcA. This work proves that α- and β-tetrasaccharide are equally potent immunogens.
    Electronic ISSN: 1420-3049
    Topics: Chemistry and Pharmacology
    Published by Molecular Diversity Preservation International
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  • 2
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    Gravity modelling of the Tyrrhenian-Calabrian-Ionian subduction system (2024)
    Frontiers S.A.
    Details
    Publication Date: 2024-02-09
    Description: This study provides a lithospheric-scale model along the Ionian Subduction zone in Southern Italy, contributing to the seismotectonic investigation of a region which is affected by relevant historical seismicity. The study employs gravity forward modelling to build the geo-structural model along a composite, NWSE trending transect extending from the Ionian to the Tyrrhenian Sea, including the Aeolian arc and the Calabro-Peloritan onshore. Through a multidisciplinary approach, we propose new interpretations of three 2D deep-seismic reflection profiles across the study area. Such interpretative profiles are used as constraints to model the observed Bouguer gravity anomalies providing upper and lower crust geometries. Whilst a tomographic model provides constraints for the lithospheric and asthenospheric modelling. The entire workflow is constrained by literature data about Moho geometry, deep seismicity and tomographic images that are integrated to determine the subducting slab geometry. The proposed model of the entire subducting system reasonably fits the observed gravity field and is coherent with the first-order geological and geophysical constraints. The modelling results in updated Tyrrhenian and Ionian Moho depth, subducting slab geometry and location, and densities of the main units, providing valuable input about the composition and geometry of the Calabrian arc structures.
    Description: PRIN-2017 (project #2017KT2MKE_003)
    Description: Published
    Description: 1259831
    Description: OST1 Alla ricerca dei Motori Geodinamici
    Description: JCR Journal
    Keywords: Calabrian Arc (Italy) ; subducion complex ; gravity forward modeling, crustal model ; Ionian Subduction zone ; Tyrrhenian back-arc basin-calabrian arc-accretionary wedge system ; Southern Italy ; 04.01. Earth Interior ; 04.07. Tectonophysics ; 04.04. Geology
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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