Publication Date:
2019
Description:
Abstract
Diapause is an important overwintering strategy of Locusta migratoria to survive under stressed conditions. We identified a novel dual‐specificity kinase gene that is differentially expressed between long and short day‐treated L. migratoria. To determine its function on photoperiodic diapause induction, we cloned the specific gene. Interestingly, phylogenetic analysis shows that this dual‐specificity kinase is a Mycetozoa Protein Kinase‐Like (MPKL) that may have been transferred horizontally from Mycetozoa to L. migratoria. RNAi results confirm that MPKL promotes photoperiodic diapause induction of L. migratoria. Furthermore, MPKL significantly inhibits Akt and FOXO phosphorylation levels in ovaries, enhances reactive oxygen species, superoxide dismutase and catalase activities, while decreasing peroxidase activity under both photoperiodic regimes. Our findings offer insights into the molecular mechanism of dual‐specificity kinase‐induced diapause in insects.
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Print ISSN:
0014-5793
Electronic ISSN:
1873-3468
Topics:
Biology
,
Chemistry and Pharmacology
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