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  • nodD3  (2)
  • Alfalfa  (1)
  • Springer  (3)
  • EMBO Press
  • Munksgaard International Publishers
  • Wiley-Blackwell
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Keywords
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  • Springer  (3)
  • EMBO Press
  • Munksgaard International Publishers
  • Wiley-Blackwell
Years
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: Chromosomal mapping and expression (1995)
    Springer
    Plant molecular biology 27 (1995), S. 1059-1070 
    Details
    ISSN: 1573-5028
    Keywords: Alfalfa ; cell division cycle ; chromosomal location ; cyclin ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclins in association with the protein kinase p34cdc2and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020880
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  • 2
    Electronic Resource
    Electronic Resource
    The Rhizobium meliloti early nodulation genes (nodABC) are nitrogen-regulated: Isolation of a mutant strain with efficient nodulation capacity on alfalfa in the presence of ammonium (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 89-96 
    Details
    ISSN: 1617-4623
    Keywords: Nitrogen regulation (ntr) ; nodABC ; nodD3 ; Nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The presence of combined nitrogen in the soil suppresses the formation of nitrogen-fixing root nodules by Rhizobium. We demonstrate that bacterial genes determining early nodulation functions (nodABC) as well as the regulatory gene nodD3 are under nitrogen (NH 4 + ) control. Our results suggest that the gene product of nodD3 has a role in mediating the ammonia regulation of early nod genes. The general nitrogen regulatory (ntr) system as well as a chromosomal locus mutated in Rhizobium meliloti were also found to be involved in the regulation of nod gene expression. A R. meliloti mutant with altered sensitivity to ammonia regulation was isolated, capable of more efficient nodulation of alfalfa than the wild-type strain in the presence of 2 mM ammonium sulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261162
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  • 3
    Electronic Resource
    Electronic Resource
    Genes at different regulatory levels are required for the ammonia control of nodulation in Rhizobium meliloti (1993)
    Springer
    Molecular genetics and genomics 240 (1993), S. 435-444 
    Details
    ISSN: 1617-4623
    Keywords: Nitrogen regulation ; nodD3 ; syrM ; Common nod genes ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The expression of the nodulation genes nodABC of Rhizobium meliloti, which determine early response functions to plant host signals, is regulated by the level of ammonia, the primary product of symbiotic nitrogen fixation. We show that the pathway that links the ammonia-induced signal to the transcriptional control of the nodABC genes involves at least two regulatory levels. The fluctuating nitrogen level is sensed and the signal is mediated by the members of the general nitrogen regulatory (ntr) system, then transmitted to the syrM-nodD3 genes representing the nod-specific level of ammonia regulation. At low ammonia concentration, the activator protein NtrC exerts its effect via nodD3. In conditions of nitrogen excess ntrR, involved in the repression of nod genes, may function in coordination with the syrM gene. Finally, the NodD3 protein may relay the nitrogen status signal to the transcriptional control of the nodABC genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00280398
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