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  • Complex I  (3)
  • 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous  (1)
  • Springer  (4)
  • EDP Sciences
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  • Springer  (4)
  • EDP Sciences
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  • 1
    Electronic Resource
    Electronic Resource
    Disruption of the nuclear gene encoding the 20.8-kDa subunit of NADH:ubiquinone reductase ofNeurospora mitochondria (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 177-183 
    Details
    ISSN: 1617-4623
    Keywords: Neurospora crassa ; Mitochondria ; Complex I ; Assembly ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nuclear gene coding for the 20.8-kDa subunit of the membrane arm of respiratory chain NADH:ubiquinone reductase (Complex I) fromNeurospora crassa, nuo-20.8, was localized on linkage group I of the fungal genome. A genomic DNA fragment containing this gene was cloned and a duplication was created in a strain ofN. crassa by transformation. To generate RIP (repeat-induced point) mutations in the duplicated sequence, the transformant was crossed with another strain carrying an auxotrophic marker on chromosome I. To increase the chance of finding an isolate with a non-functionalnuo-20.8 gene, random progeny from the cross were selected against this auxotrophy since RIP of the target gene will only occur in the nucleus carrying the duplication. Among these, we isolated and characterised a mutant strain that lacks the 20.8 kDa mitochondrial protein, indicating that this cysteine-rich polypeptide is not essential. Nevertheless, the absence of the 20.8-kDa subunit prevents the full assembly of complex I. It appears that the peripheral arm and two intermediates of the membrane arm of the enzyme are still formed in the mutant mitochondria. The NADH:ubiquinone reductase activity of sonicated mitochondria from the mutant is rotenone insensitive. Electron microscopy of mutant mitochondria does not reveal any alteration in the structure or numbers of the organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173218
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Disruption of the nuclear gene encoding the 20.8-kDa subunit of NADH:ubiquinone reductase of Neurospora mitochondria (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 177-183 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsNeurospora crassa ; Mitochondria ; Complex I ; Assembly ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nuclear gene coding for the 20.8k-kDa subunit of the membrane arm of respiratory chain NADH:ubiquinone reductase (Complex I) from Neurospora crassa, nuo-20.8 was localized on linkage group I of the fungal genome. A genomic DNA fragment containing this gene was cloned and a duplication was created in a strain of N. crassa by transformation. To generate RIP (repeat-induced point) mutations in the duplicated sequence, the transformant was crossed with another strain carrying an auxotrophic marker on chromosome I. To increase the chance of finding an isolate with a non-functional nuo-20.8 gene, random progeny from the cross were selected against this auxotrophy since RIP of the target gene will only occur in the nucleus carrying the duplication. Among these, we isolated and characterised a mutant strain that lacks the 20.8 kDa mitochondrial protein, indicating that this cysteine-rich polypeptide is not essential. Nevertheless, the absence of the 20.8-kDa subunit prevents the full assembly of complex I. It appears that the peripheral arm and two intermediates of the membrane arm of the enzyme are still formed in the mutant mitochondria. The NADH:ubiquinone reductase activity of sonicated mitochondria from the mutant is rotenone insensitive. Electron microscopy of mutant mitochondria does not reveal any alteration in the structure or numbers of the organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004389670020
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Inactivation of the gene coding for the 30.4-kDa subunit of respiratory chain NADH dehydrogenase: is the enzyme essential for Neurospora? (1998)
    Springer
    Molecular genetics and genomics 257 (1998), S. 368-375 
    Details
    ISSN: 1617-4623
    Keywords: Key words Mitochondria ; Complex I ; Gene disruption ; Mutants ; Neurospora crassa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and characterised the nuclear gene that codes for the 30.4-kDa subunit of the peripheral arm of complex I from Neurospora crassa. The single-copy gene was localised on chromosome VI of the fungal genome by restriction fragment length polymorphism mapping. An extra copy of the gene was introduced into a strain of N. crassa by transformation. This strain was crossed with another strain in order to inactivate, by repeat-induced point mutations, both copies of the duplication carried by the parental transformant. Ascospore progeny from the cross were analysed and a mutant strain lacking the 30.4-kDa protein, nuo30.4, was isolated and further characterised. The mutant appears to assemble the membrane arm of complex I, while formation of the peripheral arm is prevented. Nevertheless, the mutant grows reasonably well – indicating that this well conserved protein is not essential for vegetative growth – and is able to mate with other strains both as male or female. Strains with multiple mutations are readily obtained from heterozygous crosses between different complex I mutants of N. crassa. On the other hand, homozygous crosses between several mutants, including nuo30.4, fail to produce ascospores. These results suggest that complex I plays an essential role during the sexual phase of the life cycle of the fungus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050659
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  • 4
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    Assessing seismic damage through stochastic simulation of ground shaking: the case of the 1998 Faial Earthquake (Azores Islands) (2009)
    Springer
    Details
    Publication Date: 2020-12-23
    Description: In July 1998, an Mw = 6.2 earthquake struck the islands of Faial, Pico and San Jorge (in the Azores Archipelago), registering VIII on the Modified Mercalli Intensity scale and causing major destruction in the northeastern part of Faial. The main shock was located offshore, 8 km North East of the island, and it triggered a seismic sequence that lasted for several weeks. The existing data for this earthquake include both the general tectonic environment of the region and the teleseismic information. This is accompanied by one strong-motion record obtained 15 km from the epicentre, the epicentre location of aftershocks, and a large collection of the damage inflicted to the building stock (as poor rubble masonry, of 2-3 storeys). The present study was carried out in two steps: first, with a finite-fault stochastic simulation method of ground motion at sites throughout the affected islands, for two possible locations of the rupturing fault and for a large number of combinations of rupture mechanisms (as a parametric analysis); secondly, the damage to buildings was modelled using a well-known macroseismic method that considers the building typologies and their associated vulnerabilities. The main intent was to integrate different data (geological, seismological and building features) to produce a scenario model to reproduce and justify the level of damage generated during the Faial earthquake. Finally, through validation of the results provided by these different approaches, we obtained a complete procedure for the parameters of a first model for the production of seismic damage scenarios for the Azores Islands region.
    Description: Published
    Description: 361–381
    Description: 5T. Sismologia, geofisica e geologia per l'ingegneria sismica
    Description: JCR Journal
    Description: open
    Keywords: Stochastic finite-fault scenarios ; Building damage assessment ; 9th July Faial earthquake ; Azores Islands ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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