ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    β-Tubulin mutation suppresses microtubule dynamics in vitro and slows mitosis in vivo (1995)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 30 (1995), S. 285-300 
    Details
    ISSN: 0886-1544
    Keywords: microtubule dynamics ; β-tubulin ; mitosis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microtubule (MT) dynamics vary both spatially and temporally within cells and are thought to be important for proper MT cellular function. Because MT dynamics appear to be closely tied to the guanosine triphosphatase (GTPase) activity of β-tubulin subunits, we examined the importance of MT dynamics in the budding yeast S. cerevisiae by introducing a T107K point mutation into a region of the single β-tubulin gene, TUB2, known to affect the assembly-dependent GTPase activity of MTs in vitro. Analysis of MT dynamic behavior by video-enhanced differential interference contrast microscopy, revealed that T107K subunits slowed both the growth rates and catastrophic disassembly rates of individual MTs in vitro. In haploid cells tub2-T107K is lethal; but in tub2-T107K/tub2-590 heterozygotes the mutation is viable, dominant, and slows cell-cycle progression through mitosis, without causing wholesale disruption of cellular MTs. The correlation between the slower growing and shortening rates of MTs in vitro, and the slower mitosis in vivo suggests that MT dynamics are important in budding yeast and may regulate the rate of nuclear movement and segregation. The slower mitosis in mutant celis did not result in premature cytokinesis and cell death, further suggesting that cell-cycle control mechanisms “sense” the mitotic slowdown, possibly by monitoring MT dynamics directly. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970300406
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Automated amplification and sequencing of human mitochondrial DNA (1991)
    Weinheim : Wiley-Blackwell
    Electrophoresis 12 (1991), S. 17-21 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Part of the human mitochondrial D-loop region was amplified by two successive rounds of polymerase chain reaction (PCR) amplification. In the second PCR reaction, nested primers were used, of which one contained the M 13-21 universal primer sequence. By using nonequal concentrations of primers in the second amplification, single-stranded DNA was generated. This was then sequenced directly by the dideoxy chain termination method using dye-labelled universal sequencing primers in conjunction with a fluorescence-based DNA sequencer. This enabled a 403-base-pair hypervariable segment of the D-loop region to be readily sequenced in a single reaction. This paper describes a protocol which enables mitochondrial sequence information to be generated rapidly and automatically. It is likely to be of importance in forensic analysis where the DNA is too degraded or of insufficient quantity to be analysed by other techniques.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150120105
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A rapid polymerase chain reaction method for identifying fixed specimens (1992)
    Weinheim : Wiley-Blackwell
    Electrophoresis 13 (1992), S. 173-175 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin “Chelex” to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10-2 and 10-4. The methods described have considerable potential for use in routine genetic analysis of preserved samples.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150130135
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    facet.materialart.
    Unknown
    A 17-year dataset of surface water fugacity of CO2 along with calculated pH, aragonite saturation state and air–sea CO2 fluxes in the northern Caribbean Sea (2020)
    Copernicus
    Details
    Publication Date: 2020-07-06
    Description: A high-quality dataset of surface water fugacity of CO2 (fCO2w)1, consisting of over a million observations, and derived products are presented for the northern Caribbean Sea, covering the time span from 2002 through 2018. Prior to installation of automated pCO2 systems on cruise ships of Royal Caribbean International and subsidiaries, very limited surface water carbon data were available in this region. With this observational program, the northern Caribbean Sea has now become one of the best-sampled regions for pCO2 of the world ocean. The dataset and derived quantities are binned and averaged on a 1∘ monthly grid and are available at http://accession.nodc.noaa.gov/0207749 (last access: 30 June 2020) (https://doi.org/10.25921/2swk-9w56; Wanninkhof et al., 2019a). The derived quantities include total alkalinity (TA), acidity (pH), aragonite saturation state (ΩAr) and air–sea CO2 flux and cover the region from 15 to 28∘ N and 88 to 62∘ W. The gridded data and products are used for determination of status and trends of ocean acidification, for quantifying air–sea CO2 fluxes and for ground-truthing models. Methodologies to derive the TA, pH and ΩAr and to calculate the fluxes from fCO2w temperature and salinity are described.
    Print ISSN: 1866-3508
    Electronic ISSN: 1866-3516
    Topics: Geosciences
    Published by Copernicus
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 5
    facet.materialart.
    Unknown
    A multi-decade record of high-quality fCO2 data in version 3 of the Surface Ocean CO2 Atlas (SOCAT) (2016)
    Copernicus
    Details
    Publication Date: 2016-05-25
    Description: The Surface Ocean CO2 Atlas (SOCAT) is a synthesis of quality-controlled fCO2 (fugacity of carbon dioxide) values for the global surface oceans and coastal seas with regular updates. Version 3 of SOCAT has 14.5 million fCO2 values from 3646 data sets covering the years 1957 to 2014. This latest version has an additional 4.4 million fCO2 values relative to version 2 and extends the record from 2011 to 2014. Version 3 also significantly increases the data availability for 2005 to 2013. SOCAT has an average of approximately 1.2 million surface water fCO2 values per year for the years 2006 to 2012. Quality and documentation of the data has improved. A new feature is the data set quality control (QC) flag of E for data from alternative sensors and platforms. The accuracy of surface water fCO2 has been defined for all data set QC flags. Automated range checking has been carried out for all data sets during their upload into SOCAT. The upgrade of the interactive Data Set Viewer (previously known as the Cruise Data Viewer) allows better interrogation of the SOCAT data collection and rapid creation of high-quality figures for scientific presentations. Automated data upload has been launched for version 4 and will enable more frequent SOCAT releases in the future. High-profile scientific applications of SOCAT include quantification of the ocean sink for atmospheric carbon dioxide and its long-term variation, detection of ocean acidification, as well as evaluation of coupled-climate and ocean-only biogeochemical models. Users of SOCAT data products are urged to acknowledge the contribution of data providers, as stated in the SOCAT Fair Data Use Statement. This ESSD (Earth System Science Data) "Living Data" publication documents the methods and data sets used for the assembly of this new version of the SOCAT data collection and compares these with those used for earlier versions of the data collection (Pfeil et al., 2013; Sabine et al., 2013; Bakker et al., 2014).
    Electronic ISSN: 1866-3591
    Topics: Geosciences
    Published by Copernicus
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 6
    facet.materialart.
    Unknown
    A multi-decade record of high-quality fCO2 data in version 3 of the Surface Ocean CO2 Atlas (SOCAT) (2016)
    Copernicus
    Details
    Publication Date: 2016-09-15
    Description: The Surface Ocean CO2 Atlas (SOCAT) is a synthesis of quality-controlled fCO2 (fugacity of carbon dioxide) values for the global surface oceans and coastal seas with regular updates. Version 3 of SOCAT has 14.7 million fCO2 values from 3646 data sets covering the years 1957 to 2014. This latest version has an additional 4.6 million fCO2 values relative to version 2 and extends the record from 2011 to 2014. Version 3 also significantly increases the data availability for 2005 to 2013. SOCAT has an average of approximately 1.2 million surface water fCO2 values per year for the years 2006 to 2012. Quality and documentation of the data has improved. A new feature is the data set quality control (QC) flag of E for data from alternative sensors and platforms. The accuracy of surface water fCO2 has been defined for all data set QC flags. Automated range checking has been carried out for all data sets during their upload into SOCAT. The upgrade of the interactive Data Set Viewer (previously known as the Cruise Data Viewer) allows better interrogation of the SOCAT data collection and rapid creation of high-quality figures for scientific presentations. Automated data upload has been launched for version 4 and will enable more frequent SOCAT releases in the future. High-profile scientific applications of SOCAT include quantification of the ocean sink for atmospheric carbon dioxide and its long-term variation, detection of ocean acidification, as well as evaluation of coupled-climate and ocean-only biogeochemical models. Users of SOCAT data products are urged to acknowledge the contribution of data providers, as stated in the SOCAT Fair Data Use Statement. This ESSD (Earth System Science Data) "living data" publication documents the methods and data sets used for the assembly of this new version of the SOCAT data collection and compares these with those used for earlier versions of the data collection (Pfeil et al., 2013; Sabine et al., 2013; Bakker et al., 2014). Individual data set files, included in the synthesis product, can be downloaded here: doi:10.1594/PANGAEA.849770. The gridded products are available here: doi:10.3334/CDIAC/OTG.SOCAT_V3_GRID.
    Print ISSN: 1866-3508
    Electronic ISSN: 1866-3516
    Topics: Geosciences
    Published by Copernicus
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 7
    facet.materialart.
    Unknown
    SURATLANT: a 1993–2017 surface sampling in the central part of the North Atlantic subpolar gyre (2018)
    Copernicus
    Details
    Publication Date: 2018-10-18
    Description: This paper presents the SURATLANT data set (SURveillance ATLANTique). It consists of individual data of temperature, salinity, parameters of the carbonate system, nutrients, and water stable isotopes (δ18O and δD) collected mostly from ships of opportunity since 1993 along transects between Iceland and Newfoundland (https://doi.org/10.17882/54517). We discuss how the data are validated and qualified, their accuracy, and the overall characteristics of the data set. The data are used to reconstruct seasonal cycles and interannual anomalies, in particular of sea surface salinity (SSS); inorganic nutrients; dissolved inorganic carbon (DIC); and its isotopic composition δ13CDIC, total alkalinity (At), and water isotope concentrations. Derived parameters such as fCO2 and pH are also estimated. The relation between salinity and At is estimated from these data to investigate the possibility to replace missing At when estimating other parameters of the carbonate system. When examining the average seasonal cycle in the deep ocean, in both these data with other climatologies, we find a period of small seasonal change between January and late April. On the Newfoundland shelf and continental slope, changes related with spring stratification and blooms occur earlier. The data were collected in a period of multi-decennial variability associated with the Atlantic multi-decadal variability with warming between 1994 and 2004–2007, and with the recent cooling having peaked in 2014–2016. We also observe strong salinification in 2004–2009 and fresher waters in 1994–1995 as well as since 2010 south of 54° N and in 2016–2017 north of 54° N. Indication of multi-decadal variability is also suggested by other variables, such as phosphate or DIC, but cannot be well resolved seasonally with the discrete sampling and in the presence of interannual variability. As a whole, over the 24 years, the ocean fCO2 trend (+1.9 µatm yr−1) is close to the atmospheric trend and associated with an increase in DIC (+0.77 µmol kg−1 yr−1). The data also revealed a canonical pH decrease of −0.0021 yr−1. There is also a decrease in δ13CDIC between 2005 and 2017 (in winter, −0.014 ‰ yr−1, but larger in summer, −0.042 ‰ yr−1), suggesting a significant anthropogenic carbon signal at play together with other processes (mixing, biological activity).
    Print ISSN: 1866-3508
    Electronic ISSN: 1866-3516
    Topics: Geosciences
    Published by Copernicus
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 8
    facet.materialart.
    Unknown
    SURATLANT: a 1993–2017 surface sampling in the central part of the North Atlantic subpolar gyre (2018)
    Copernicus
    Details
    Publication Date: 2018-04-24
    Description: This paper presents the SURATLANT dataset (SURveillance ATLANTique), consisting of individual data of temperature, salinity, parameters of the carbonate system, nutrients and water stable isotopes (δ18O and δD) collected mostly from ships of opportunity since 1993 along transects between Iceland and Newfoundland (https://doi.org/10.17882/54517). We discuss how the data are validated, qualified, their accuracy and the overall characteristics of the data set. The data are used to reconstruct seasonal cycles and interannual anomalies, in particular of Sea Surface Salinity (SSS), inorganic nutrients, dissolved inorganic carbon (DIC) and its isotopic composition δ13CDIC, total alkalinity (At), and water isotope concentrations. Derived parameters, such as fCO2 and pH are also estimated. The relation between salinity and At is estimated in these data to investigate the possibility to replace missing At when estimating other parameters of the carbonate system. We compare the seasonal cycle derived from these data with other climatologies. We find a period of small seasonal change between January and late April, except on the Newfoundland shelf/continental slope, when changes related with spring-stratification and blooms occur earlier. The data were collected in a period of multi-decennial variability associated with the Atlantic meridional oscillation with warming between 1994 and 2004–2007, and the recent cooling having peaked in 2014–2016. We also observe strong salinification in 2004–2009 and fresher waters in 1994–1995 as well as since 2010 south of 54°N and in 2016–2017 north of 54°N. Indication of multi-decadal variability is also suggested by other variables, such as phosphate or DIC, but cannot be well resolved seasonally with the discrete sampling and in the presence of interannual variability. As a whole, over the 24 years ocean fCO2 trend (+1.9µatmyr-1) is close to the atmospheric trend and associated with an increase in DIC (+0.70μmolkg-1yr-1). The data also revealed a "canonical" pH decrease of −0.002yr-1. There is also a decrease in δ13CDIC between 2005 and 2017 (in winter, −0.015‰yr-1, but larger in summer, −0.042‰yr-1), suggesting significant anthropogenic carbon signal at play together with other processes (mixing, biological activity).
    Electronic ISSN: 1866-3591
    Topics: Geosciences
    Published by Copernicus
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...