Keywords:
Proteins .
;
Biotechnology.
;
Biology Technique.
;
Protein Biochemistry.
;
Chemical Bioengineering.
;
Biological Techniques.
Description / Table of Contents:
Literature Search -- Sequence Analysis -- Structural Analysis -- Protein Expression Hosts and Expression Plasmids -- Gene Cloning -- Protein Expression -- Assay -- Gene Mutagenesis -- High-Throughput Screening (HTS) -- Protein Variants Analysis and Characterization -- Continuing from Protein Variants -- Employability.
Abstract:
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Type of Medium:
Online Resource
Pages:
XV, 202 p. 91 illus., 68 illus. in color.
,
online resource.
Edition:
1st ed. 2020.
ISBN:
9783030568986
Series Statement:
Learning Materials in Biosciences,
URL:
https://doi.org/10.1007/978-3-030-56898-6
DOI:
10.1007/978-3-030-56898-6
DDC:
572.6
Language:
English
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