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  • Springer  (1,276)
  • Cell Press  (171)
  • Wiley-Blackwell  (106)
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  • 1
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    Effect of SiC nanoparticles on the precipitation behavior and mechanical properties of 7075Al alloy (2020)
    Springer
    Details
    Publication Date: 2020-02-04
    Print ISSN: 0022-2461
    Electronic ISSN: 1573-4803
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Published by Springer
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Cyclic AMP induces rapid increases in gap junction permeability and changes in the cellular distribution of connexin43 (1995)
    Springer
    The journal of membrane biology 148 (1995), S. 243-253 
    Details
    ISSN: 1432-1424
    Keywords: Gap junction ; Connexin43 (Cx43) ; Cyclic AMP ; Fluorescence recovery after photobleaching ; Junctional communication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The rapid effects of cAMP on gap junction-mediated intercellular communication were examined in several cell types which express different levels of the gap junction protein, connexin43 (Cx43), including immortalized rat hepatocyte and granulosa cells, bovine coronary venular endothelial cells, primary rat myometrial and equine uterine epithelial cells. Functional analysis of changes in junctional communication induced by 8-bromo-cAMP was monitored by a fluorescence recovery after photobleaching assay in subconfluent cultures in the presence or absence of 1.0 mm 1-octanol (an agent which uncouples cells by closing gap junction channels). Communicating cells treated with 1.0 mm 8-bromo-cAMP alone exhibited significant increases in the percent of fluorescence recovery which were detected within 1–3 min depending on cell type, and junctional communication remained significantly elevated for up to 24 hr. Addition of 1.0 mm 8-bromo-cAMP to cultured cells, which were uncoupled with 1.0 mm octanol for 1 min, exhibited partial restoration of gap junctional permeability beginning within 3–5 min. Identical treatments were performed on cultures that were subsequently processed for indirect immunofluorescence to monitor Cx43 distribution. The changes in junctional permeability of cells correlated with changes in the distribution of immunoreactive Cx43. Cells treated for 2 hr with 10 μm monensin exhibited a reduced communication rate which was accompanied by increased vesicular cytoplasmic Cx43 staining and reduced punctate surface staining of junctional plaques. Addition of 1.0 mm 8-bromo-cAMP to these cultures had no effect on the rate of communication or the distribution of Cx43 compared to cultures treated with monensin alone. These data suggest that an effect of cyclic AMP on Cx43 gap junctions is to promote increases in gap junctional permeability by increasing trafficking and/or assembly of Cx43 to plasma membrane gap junctional plaques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235042
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  • 3
    Electronic Resource
    Electronic Resource
    H+ transport and the regulation of intracellular pH in ehrlich ascites tumor cells (1984)
    Springer
    The journal of membrane biology 79 (1984), S. 7-18 
    Details
    ISSN: 1432-1424
    Keywords: Ehrlich cells ; intracellular pH ; pH regulation ; H+ transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The intracellular pH (pH i ) of Ehrlich ascites tumor cells, both in the steady state and under conditions of acid loading or recovery from acid loading, was investigated by measuring the transmembrane flux of H+ equivalents and correlating this with changes in the distribution ratio of dimethyloxazolidine-2,4-dione (DMO). The pH i of cells placed in an acidic medium (pH o below 7.15) decreases and reaches a steady-state value that is more alkaline than the outside. For example when pH o is acutely reduced to 5.5, pH i falls exponentially from 7.20 ± 0.06 to 6.29 ± 0.04 with a halftime of 5.92 ± 1.37 min, suggesting a rapid influx of H+. The unidirectional influx of H+ exhibits saturation kinetics with respect to extracellular [H+]; the maximal flux is 15.8 ± 0.05 mmol/(kg dry wt · min) andK m is 0.74 ± 0.09 × 10−6 m. Steady-state cells with pH i above 6.8 continuously extrude H+ by a process that is not dependent on ATP but is inhibited by anaerobiosis. Acid-loaded cells (pH i 6.3) when returned to pH o 7.3 medium respond by transporting H+, resulting in a rapid rise in pH i . The halftime for this process is 1.09 ± 0.22 min. The H+ efflux measured under similar conditions increases as the intracellular acid load increases. An ATP-independent as well as an ATP-dependent efflux contributes to the restoration of pH i to its steady-state value.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868522
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  • 4
    Electronic Resource
    Electronic Resource
    Furosemide Stimulates K Transport in HCD57 Erythroid Cells (2000)
    Springer
    The journal of membrane biology 175 (2000), S. 235-244 
    Details
    ISSN: 1432-1424
    Keywords: Key words: Furosemide — K transport — Serum-deprivation — Cell shrinkage — HCD57 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract. We examined the influence of serum and furosemide on K movement and cell volume in HCD57 cells, a murine erythroleukemia cell line, which require erythropoietin (EPO) for survival. We found that maintenance of cell volume depends on the concentration of serum in the culture medium. In isotonic medium containing 20% serum, HCD57 cells maintain their steady-state volume. In contrast, the cells shrink progressively as medium serum content is reduced. In serum-free medium, raising external K to 75 mm prevents cell shrinkage and a further increase in K to 145 mm results in swelling, revealing a role for K permeability in the regulation of cell volume. Of particular interest has been a serendipitous finding with furosemide. Below an external K concentration of 2.1 ± 0.3 mm in medium containing 2% serum, furosemide inhibits K uptake, probably stemming from its well known inhibitory action on KCl cotransport. However, above that K concentration, furosemide stimulates K uptake in a dose-dependent manner. Moreover, furosemide potentiates cell shrinkage induced by serum withdrawal. These findings suggest that the transport machinery mediating cellular shrinkage, once primed by serum depletion, becomes receptive to a second stimulus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00232001071
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  • 5
    Electronic Resource
    Electronic Resource
    Evidence for titratable gating charges controlling the voltage dependence of the outer mitochondrial membrane channel, VDAC (1985)
    Springer
    The journal of membrane biology 86 (1985), S. 51-59 
    Details
    ISSN: 1432-1424
    Keywords: membrane protein ; pore ; protein modification ; gating mechanism ; planar bilayer membrane ; protein titration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A voltage-dependent anion-selective channel, VDAC, is found in outer mitochondrial membranes. VDAC's conductance is known to decrease as the transmembrane voltage is increased in either the positive or negative direction. Charged groups on the channel may be responsible for this voltage dependence by allowing the channel to respond to an applied electric field. If so, then neutralization of these charges would eliminate the voltage dependence. Channels in planar lipid bilayers which behaved normally at pH 6 lost much of their voltage dependence at high pH. Raising the pH reduced the steepness of the voltage dependence and raised the voltage needed to close half the channels. In contrast, the energy difference between the open and closed state in the absence of a field was changed very little by the elevated pH. The groups being titrated had an apparent pK of 10.6. From the pK and chemical modification, lysine epsilon amino groups are the most likely candidates responsible for VDAC's ability to respond to an applied electric field.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01871610
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  • 6
    Electronic Resource
    Electronic Resource
    Mössbauer study of the thermal decomposition of lepidocrocite and characterization of the decomposition products (1991)
    Springer
    Physics and chemistry of minerals 18 (1991), S. 131-143 
    Details
    ISSN: 1432-2021
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract The lepidocrocite (γ-FeOOH) to maghemite (γ-Fe2O3), and the maghemite to hematite (α-Fe2O3) transition temperatures have been monitored by TGA and DSC measurements for four initial γ-FeOOH samples with different particle sizes. The transition temperature of γ-FeOOH to γ-Fe2O3 and the size of the resulting particles were not affected by the particle size of the parent lepidocrocite. In contrast, the γ-Fe2O3 to γ-Fe2O3 transition temperature seems to depend on the amount of excess water molecules present in the parent lepidocrocite. Thirteen products obtained by heating for one hour at selected temperatures, were considered. Powder X-ray diffraction was used to qualify their composition and to determine their mean crystallite diameters. Transmission electron micrographs revealed the particle morphology. The Mössbauer spectra at 80 K and room temperature of the mixed and pure decomposition products generally had to be analyzed with a distribution of hyperfine fields and, where appropriate, with an additional quadrupole-splitting distribution. The Mössbauer spectra at variable temperature between 4.2 and 400 K of two single-phase γ-Fe2O3 samples with extremely small particles show the effect of superparamagnetism over a very broad temperature range. Only at the lowest temperatures (T⩽55 K), two distributed components were resolved from the magnetically split spectra. In the external-field spectra the ΔmI=0 transitions have not vanished. This effect is an intrinsic property of the maghemite particles, indicating a strong spin canting with respect to the applied-field direction. The spectra are successfully reproduced using a bidimensional-distribution approach in which both the canting angle and the magnetic hyperfine field vary within certain intervals. The observed distributions are ascribed to the defect structure of the maghemites (unordered vacancy distribution on B-sites, large surface-to-bulk ratio, presence of OH- groups). An important new finding is the correlation between the magnitude of the hyperfine field and the average canting angle for A-site ferric ions, whereas the B-site spins show a more uniform canting. The Mössbauer parameters of the two hematite samples with MCD104 values of respectively 61.0 and 26.5 nm display a temperature variation which is very similar to that of small-particle hematites obtained from thermal decomposition of goethite. However, for a given MCD the Morin transition temperature for the latter samples is about 30 K lower. This has tentatively been ascribed to the different mechanisms of formation, presumably resulting in slightly larger lattice parameters for the hematite particles formed from goethite, thus shifting the Morin transition to lower temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216606
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  • 7
    Electronic Resource
    Electronic Resource
    Temperature dependence of the hyperfine parameters of maghemite and al-substituted maghemites (1995)
    Springer
    Physics and chemistry of minerals 22 (1995), S. 178-185 
    Details
    ISSN: 1432-2021
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Abstract Synthetic aluminum-substituted maghemite samples, γ-(Al y Fe1-y )2O3 with y=0, 0.032, 0.058, 0.084, 0.106 and 0.151 have been studied by Mössbauer spectroscopy at 8 K and in the range 80 K to 475 K at steps of 25 K. The spectra have been analysed as a superposition of two sextets composed of asymmetrical Lorentzians. The A- and B-site isomer shifts were constrained as: δA=δB-0.12 mm/s. From the temperature dependence of δB it was possible to determine the characteristic Mössbauer temperature and the intrinsic shift. Both quantities clearly increase with increasing Al content, at least up to 10 mole%. The temperature dependence of the A-and B-sites hyperfine fields could be satisfactorily reproduced using the molecular-field theory assuming an antiparallel spin configuration. The exchange integrals were found as: J AB =-25 K; J AA =-18 K and J BB = -3 K. The hyperfine fields show a crossing in the vicinity of 300 K as a result of the relatively strong A-A interaction. The Curie temperature for the non-substituted sample was calculated to be 930 K and decreases to 765 K for the sample with 15 mol% Al. The gradual decrease of the saturation value of the A-site hyperfine field with increasing Al substitution and the constancy of this quantity for the B sites, suggest that the Al cations occupy the B sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202298
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  • 8
    Electronic Resource
    Electronic Resource
    Evidence for gibberellin-like substances in phloem exudate of higher plants (1968)
    Springer
    Planta 82 (1968), S. 22-32 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Samples of sieve-tube sap were obtained as honeydew from aphids feeding on three species of higher plants. The honeydew was extracted, chromatographed and tested in several bioassays for the presence of gibberellin-like substances. The bioassay results indicated that gibberellin-like substances were translocated in the phloem of dandelion (Taraxacum officinale), broad bean (Vicia faba) and willow (Salix viminalis). Results obtained with willow showed that the concentration of gibberellin-like substances in the sieve-tube sap is daylength dependent, high levels being present in plants maintained under long days and low levels in short day plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384695
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  • 9
    Electronic Resource
    Electronic Resource
    The interchange of 14C-kinetin and 14C-gibberellic acid between the bark and xylem of willow (1969)
    Springer
    Planta 89 (1969), S. 108-125 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Considerable two-way interchange of label derived from 14C-GA3 and 14C-kinetin has been shown to occur between the xylem and extra-cambial tissue of Salix viminalis L. Radial translocation of both growth substances from the transpiration stream to the sieve-tube sap has been demonstrated by the use of an aphid technique. This is the first record of the bark-xylem interchange of these two growth hormones. A high percentage of 14C-label is present in compounds other than GA3 and kinetin 8 hours after application. Mechanisms of lateral transfer are discussed together with their possible physiological significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386979
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  • 10
    Electronic Resource
    Electronic Resource
    Direct analysis of RNA transcripts in electroporated carrot protoplasts (1990)
    Springer
    Plant cell reports 9 (1990), S. 129-132 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a method for direct analysis of RNA transcribed from DNA introduced into carrot cells by electroporation. Octopine synthase RNA transcribed from the plasmid p35SOcs was detected in total and poly A+ RNA on Northern blots and in RNA protection assays. The highest level of octopine synthase transcript was detected at approximately 8 hrs post-electroporation, although RNA could still be detected after 48 hrs. This method allows detection of foreign gene expression in a plant system and bypasses the need for reporter genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232087
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